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100 1 _ |a Berkamp, Sabrina
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245 _ _ |a High-Throughput Correlative Light and Cryo-Electron Microscopy Pipeline Using PRIMO Micropatterning, CERES Ice Shield and the METEOR In-Chamber Fluorescence Light Microscope
260 _ _ |a Oxford
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520 _ _ |a Correlative light and cryo-electron microscopy (CLEM) can be used to study macromolecular structures in vitreous state of the complex environment of the cell. The associated workflow, however, is still cumbersome and low throughput. It includes many steps and is therefore prone to ice contamination, cracked lamellae or devitrification all of which can result in sample loss. We have implemented several technological improvements to the workflow resulting in a much simpler and higher throughput method, yielding more lamellae with reduced ice contamination.
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700 1 _ |a Smeets, Marit
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700 1 _ |a Caignard, Alexane
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700 1 _ |a Jani, Riddhi
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700 1 _ |a Daviran, Deniz
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700 1 _ |a Sachse, Carsten
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773 _ _ |a 10.1093/micmic/ozad067.521
|g Vol. 29, no. Supplement_1, p. 1027 - 1028
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|t Microscopy and microanalysis
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