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@ARTICLE{Verspreet:1025121,
      author       = {Verspreet, Joran and Kuchendorf, Christina and Ackermann,
                      Bärbel and Bastiaens, Leen},
      title        = {{T}he {I}mpact of {N}utrient {L}imitation and {H}arvest
                      {M}ethod on the {W}et {P}reservation of {C}hlorella vulgaris
                      {B}iomass},
      journal      = {Bioengineering},
      volume       = {10},
      number       = {5},
      issn         = {2306-5354},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2024-02708},
      pages        = {600 -},
      year         = {2023},
      note         = {The applicable Grant was North-West Europe Interreg, grant
                      number NWE 639},
      abstract     = {The temporary storage of wet algae concentrates enables the
                      decoupling in time of algae harvests and their biorefinery.
                      However, the impact of cultivation and of the harvest
                      conditions on algae quality during preservation is largely
                      unknown. This study aimed to determine the impact of
                      nutrient limitation and of harvest methods on the
                      preservation of Chlorella vulgaris biomass. Algae were
                      either well-fed until harvest or received no nutrients for
                      one week, and were harvested by either batch or continuous
                      centrifugation. The organic acid formation, lipid levels,
                      and lipolysis were monitored. Nutrient limitation had a
                      large impact and resulted in lower pH values (4.9 ± 0.4),
                      high levels of lactic acid and acetic acid, and a slightly
                      higher degree of lipid hydrolysis. Concentrates of well-fed
                      algae had a higher pH (7.4 ± 0.2) and another pattern of
                      fermentation products with mainly acetic acid, succinic
                      acid, and, to a smaller extent, lactic acid and propionic
                      acid. The effect of the harvest method was smaller, with,
                      most often, higher lactic acid and acetic acid levels for
                      algae harvested by continuous centrifugation than for those
                      obtained by batch centrifugation. In conclusion, nutrient
                      limitation, a well-known method to enhance algae lipid
                      levels, can impact several quality attributes of algae
                      during their wet storage.},
      cin          = {IBG-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {37237670},
      UT           = {WOS:000995645700001},
      doi          = {10.3390/bioengineering10050600},
      url          = {https://juser.fz-juelich.de/record/1025121},
}