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@INPROCEEDINGS{Rudani:1025123,
author = {Rudani, Binny and Jakubowski, Andre and Wiegand, Simone},
title = {{T}hermodiffusion of {A}queous {S}olutions of {G}uanidinium
{S}alts},
reportid = {FZJ-2024-02710},
year = {2024},
abstract = {Thermodiffusion, or the Ludwig-Soret effect, refers to the
motion of solute molecules induced by a thermal gradient.
This phenomenon is quantified by the Soret coefficient (ST),
in aqueous solutions ST is highly sensitive to the nature of
solute-water interactions. It is being used as a tool to
probe molecular interactions, mainly protein-ligand binding.
Guanidinium ions play a pivotal role in the exploration of
protein folding, denaturation, and the hydration of
biological molecules owing to its rigid and symmetric
structure. Despite its significance, there is a dearth of
information on their thermophoretic behaviour. Recent
research by our group has delved into the thermodiffusion of
similar non-ionic organic solutes and simple ionic inorganic
salts in aqueous solutions, revealing distinctive
temperature and concentration dependencies of the ST. In our
present research, we utilize thermal diffusion forced
Rayleigh scattering (TDFRS) to scrutinize guanidinium salt
solution with various anions (carbonate, chloride, iodide
and thiocyanate), spanning the entire range of the
Hofmeister series from hydrophilic to hydrophobic. We
performed systematic measurements of ST in a temperature
range of 15 to 35°C and concentrations ranging from 0.5m to
3 m. The change of ST withtemperature as a function of
increasing concentration becomes weaker for aqueous
guanidinium salt solutions. We assume that fewer hydrogen
bonds are present at higher concentrations reducing the
change of the Soret coefficient and indicatingthat the
hydration of the salt plays an important role. Overall, this
systematic study helps to gain a fundamental understanding
of thermodiffusion in protein-ligand systems.},
month = {Mar},
date = {2024-03-25},
organization = {Bunsen-Tagung 2024, Aachen (Germany),
25 Mar 2024 - 27 Mar 2024},
subtyp = {Other},
cin = {IBI-4},
cid = {I:(DE-Juel1)IBI-4-20200312},
pnm = {5241 - Molecular Information Processing in Cellular Systems
(POF4-524)},
pid = {G:(DE-HGF)POF4-5241},
typ = {PUB:(DE-HGF)24},
doi = {10.34734/FZJ-2024-02710},
url = {https://juser.fz-juelich.de/record/1025123},
}