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@ARTICLE{Sicoli:1025262,
      author       = {Sicoli, Giuseppe and Sieme, Daniel and Overkamp, Kerstin
                      and Khalil, Mahdi and Backer, Robin and Griesinger,
                      Christian and Willbold, Dieter and Rezaei-Ghaleh, Nasrollah},
      title        = {{L}arge dynamics of a phase separating
                      arginine-glycine-rich domain revealed via nuclear and
                      electron spins},
      journal      = {Nature Communications},
      volume       = {15},
      number       = {1},
      issn         = {2041-1723},
      address      = {[London]},
      publisher    = {Nature Publishing Group UK},
      reportid     = {FZJ-2024-02814},
      pages        = {1610},
      year         = {2024},
      abstract     = {Liquid-liquid phase separation is the key process
                      underlying formation of membrane-less compartments in cells.
                      A highly dynamic cellular body with rapid component exchange
                      is Cajal body (CB), which supports the extensive
                      compositional dynamics of the RNA splicing machinery,
                      spliceosome. Here, we select an arginine-glycine (RG)-rich
                      segment of coilin, the major component of CB, establish its
                      RNA-induced phase separation, and through combined use of
                      nuclear magnetic resonance (NMR) and electron paramagnetic
                      resonance (EPR) probes, interrogate its dynamics within the
                      crowded interior of formed droplets. Taking advantage of
                      glycine-based singlet-states, we show that glycines retain a
                      large level of sub-nanoseconds dynamics inside the coilin
                      droplets. Furthermore, the continuous-wave (CW) and
                      electron-electron dipolar (PELDOR) and electron-nucleus
                      hyperfine coupling EPR data (HYSCORE) support the
                      RNA-induced formation of dynamic coilin droplets with high
                      coilin peptide concentrations. The combined NMR and EPR data
                      reveal the high dynamics of the RG-rich coilin within
                      droplets and suggest its potential role in the large
                      dynamics of CBs.},
      cin          = {IBI-7},
      ddc          = {500},
      cid          = {I:(DE-Juel1)IBI-7-20200312},
      pnm          = {5244 - Information Processing in Neuronal Networks
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5244},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {38383529},
      UT           = {WOS:001173879300032},
      doi          = {10.1038/s41467-024-45788-w},
      url          = {https://juser.fz-juelich.de/record/1025262},
}