%0 Journal Article
%A Marin, Egor
%A Kovalev, Kirill
%A Poelman, Therese
%A Veenstra, Rick
%A Borshchevskiy, Valentin
%A Guskov, Albert
%T Custom Design of a Humidifier Chamber for In Meso Crystallization
%J Crystal growth & design
%V 24
%N 1
%@ 1528-7483
%C Washington, DC
%I ACS Publ.
%M FZJ-2024-02870
%P 325 - 330
%D 2024
%X Membrane proteins are indispensable for every living organism, yet their structural organization remains underexplored. Despite the recent advancements in single-particle cryogenic electron microscopy and cryogenic electron tomography, which have significantly increased the structural coverage of membrane proteins across various kingdoms, certain scientific methods, such as time-resolved crystallography, still mostly rely on crystallization techniques, such as lipidic cubic phase (LCP) or in meso crystallization. In this study, we present an open-access blueprint for a humidity control chamber designed for LCP/in meso crystallization experiments using a Gryphon crystallization robot. Using this chamber, we have obtained crystals of a transmembrane aspartate transporter GltTk from Thermococcus kodakarensis in a lipidic environment using in meso crystallization. The data collected from these crystals allowed us to perform an analysis of lipids bound to the transporter. With this publication of our open-access design of a humidity chamber, we aim to improve the accessibility of in meso protein crystallization for the scientific community.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ 38188264
%U <Go to ISI:>//WOS:001136346000001
%R 10.1021/acs.cgd.3c01034
%U https://juser.fz-juelich.de/record/1025411