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001025411 1001_ $$00000-0003-2369-1732$$aMarin, Egor$$b0
001025411 245__ $$aCustom Design of a Humidifier Chamber for In Meso Crystallization
001025411 260__ $$aWashington, DC$$bACS Publ.$$c2024
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001025411 520__ $$aMembrane proteins are indispensable for every living organism, yet their structural organization remains underexplored. Despite the recent advancements in single-particle cryogenic electron microscopy and cryogenic electron tomography, which have significantly increased the structural coverage of membrane proteins across various kingdoms, certain scientific methods, such as time-resolved crystallography, still mostly rely on crystallization techniques, such as lipidic cubic phase (LCP) or in meso crystallization. In this study, we present an open-access blueprint for a humidity control chamber designed for LCP/in meso crystallization experiments using a Gryphon crystallization robot. Using this chamber, we have obtained crystals of a transmembrane aspartate transporter GltTk from Thermococcus kodakarensis in a lipidic environment using in meso crystallization. The data collected from these crystals allowed us to perform an analysis of lipids bound to the transporter. With this publication of our open-access design of a humidity chamber, we aim to improve the accessibility of in meso protein crystallization for the scientific community.
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001025411 7001_ $$aKovalev, Kirill$$b1
001025411 7001_ $$0P:(DE-HGF)0$$aPoelman, Therese$$b2
001025411 7001_ $$0P:(DE-HGF)0$$aVeenstra, Rick$$b3
001025411 7001_ $$0P:(DE-Juel1)144613$$aBorshchevskiy, Valentin$$b4
001025411 7001_ $$00000-0003-2340-2216$$aGuskov, Albert$$b5$$eCorresponding author
001025411 773__ $$0PERI:(DE-600)2048329-6$$a10.1021/acs.cgd.3c01034$$gVol. 24, no. 1, p. 325 - 330$$n1$$p325 - 330$$tCrystal growth & design$$v24$$x1528-7483$$y2024
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