TY  - JOUR
AU  - Marin, Egor
AU  - Kovalev, Kirill
AU  - Poelman, Therese
AU  - Veenstra, Rick
AU  - Borshchevskiy, Valentin
AU  - Guskov, Albert
TI  - Custom Design of a Humidifier Chamber for In Meso Crystallization
JO  - Crystal growth & design
VL  - 24
IS  - 1
SN  - 1528-7483
CY  - Washington, DC
PB  - ACS Publ.
M1  - FZJ-2024-02870
SP  - 325 - 330
PY  - 2024
AB  - Membrane proteins are indispensable for every living organism, yet their structural organization remains underexplored. Despite the recent advancements in single-particle cryogenic electron microscopy and cryogenic electron tomography, which have significantly increased the structural coverage of membrane proteins across various kingdoms, certain scientific methods, such as time-resolved crystallography, still mostly rely on crystallization techniques, such as lipidic cubic phase (LCP) or in meso crystallization. In this study, we present an open-access blueprint for a humidity control chamber designed for LCP/in meso crystallization experiments using a Gryphon crystallization robot. Using this chamber, we have obtained crystals of a transmembrane aspartate transporter GltTk from Thermococcus kodakarensis in a lipidic environment using in meso crystallization. The data collected from these crystals allowed us to perform an analysis of lipids bound to the transporter. With this publication of our open-access design of a humidity chamber, we aim to improve the accessibility of in meso protein crystallization for the scientific community.
LB  - PUB:(DE-HGF)16
C6  - 38188264
UR  - <Go to ISI:>//WOS:001136346000001
DO  - DOI:10.1021/acs.cgd.3c01034
UR  - https://juser.fz-juelich.de/record/1025411
ER  -