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@ARTICLE{Marin:1025411,
      author       = {Marin, Egor and Kovalev, Kirill and Poelman, Therese and
                      Veenstra, Rick and Borshchevskiy, Valentin and Guskov,
                      Albert},
      title        = {{C}ustom {D}esign of a {H}umidifier {C}hamber for {I}n
                      {M}eso {C}rystallization},
      journal      = {Crystal growth $\&$ design},
      volume       = {24},
      number       = {1},
      issn         = {1528-7483},
      address      = {Washington, DC},
      publisher    = {ACS Publ.},
      reportid     = {FZJ-2024-02870},
      pages        = {325 - 330},
      year         = {2024},
      abstract     = {Membrane proteins are indispensable for every living
                      organism, yet their structural organization remains
                      underexplored. Despite the recent advancements in
                      single-particle cryogenic electron microscopy and cryogenic
                      electron tomography, which have significantly increased the
                      structural coverage of membrane proteins across various
                      kingdoms, certain scientific methods, such as time-resolved
                      crystallography, still mostly rely on crystallization
                      techniques, such as lipidic cubic phase (LCP) or in meso
                      crystallization. In this study, we present an open-access
                      blueprint for a humidity control chamber designed for LCP/in
                      meso crystallization experiments using a Gryphon
                      crystallization robot. Using this chamber, we have obtained
                      crystals of a transmembrane aspartate transporter GltTk from
                      Thermococcus kodakarensis in a lipidic environment using in
                      meso crystallization. The data collected from these crystals
                      allowed us to perform an analysis of lipids bound to the
                      transporter. With this publication of our open-access design
                      of a humidity chamber, we aim to improve the accessibility
                      of in meso protein crystallization for the scientific
                      community.},
      cin          = {IBI-7},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBI-7-20200312},
      pnm          = {5241 - Molecular Information Processing in Cellular Systems
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {38188264},
      UT           = {WOS:001136346000001},
      doi          = {10.1021/acs.cgd.3c01034},
      url          = {https://juser.fz-juelich.de/record/1025411},
}