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001     1025411
005     20250204113841.0
024 7 _ |a 10.1021/acs.cgd.3c01034
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024 7 _ |a 10.34734/FZJ-2024-02870
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100 1 _ |a Marin, Egor
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245 _ _ |a Custom Design of a Humidifier Chamber for In Meso Crystallization
260 _ _ |a Washington, DC
|c 2024
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520 _ _ |a Membrane proteins are indispensable for every living organism, yet their structural organization remains underexplored. Despite the recent advancements in single-particle cryogenic electron microscopy and cryogenic electron tomography, which have significantly increased the structural coverage of membrane proteins across various kingdoms, certain scientific methods, such as time-resolved crystallography, still mostly rely on crystallization techniques, such as lipidic cubic phase (LCP) or in meso crystallization. In this study, we present an open-access blueprint for a humidity control chamber designed for LCP/in meso crystallization experiments using a Gryphon crystallization robot. Using this chamber, we have obtained crystals of a transmembrane aspartate transporter GltTk from Thermococcus kodakarensis in a lipidic environment using in meso crystallization. The data collected from these crystals allowed us to perform an analysis of lipids bound to the transporter. With this publication of our open-access design of a humidity chamber, we aim to improve the accessibility of in meso protein crystallization for the scientific community.
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700 1 _ |a Kovalev, Kirill
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700 1 _ |a Poelman, Therese
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700 1 _ |a Veenstra, Rick
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700 1 _ |a Borshchevskiy, Valentin
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700 1 _ |a Guskov, Albert
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|e Corresponding author
773 _ _ |a 10.1021/acs.cgd.3c01034
|g Vol. 24, no. 1, p. 325 - 330
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|t Crystal growth & design
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856 4 _ |y OpenAccess
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