TY  - JOUR
AU  - Sanyasi, Chandrasekar
AU  - Balakrishnan, Susmida Seni
AU  - Chinnasamy, Thirunavukkarasu
AU  - Venugopalan, Nagarajan
AU  - Kandavelu, Palani
AU  - Batra-Safferling, Renu
AU  - Muthuvel, Suresh Kumar
TI  - Insights on the dynamic behavior of protein disulfide isomerase in the solution environment through the SAXS technique
JO  - In Silico Pharmacology
VL  - 12
IS  - 1
SN  - 2193-9616
CY  - Heidelberg [u.a.]
PB  - SpringerOpen
M1  - FZJ-2024-03003
SP  - 23
PY  - 2024
AB  - The dynamic behavior of Protein Disulfide Isomerase (PDI) in an aqueous solution environment under physiologically active pH has been experimentally verified in this study using Small Angle X-ray Scattering (SAXS) technique. The structural mechanism of dimerization for full-length PDI molecules and co-complex with two renowned substrates has been comprehensively discussed. The structure models obtained from the SAXS data of PDI purified from bovine liver display behavior duality between unaccompanied-enzyme and after engaged with substrates. The analysis of SAXS data revealed that PDI exists as a homo-dimer in the solution environment, and substrate induction provoked its segregation into monomer to enable the enzyme to interact systematically with incoming clients.
LB  - PUB:(DE-HGF)16
DO  - DOI:10.1007/s40203-024-00198-0
UR  - https://juser.fz-juelich.de/record/1025609
ER  -