% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Engilberge:1025965,
      author       = {Engilberge, Sylvain and Caramello, Nicolas and Bukhdruker,
                      Sergei and Byrdin, Martin and Giraud, Thierry and Jacquet,
                      Philippe and Scortani, Damien and Biv, Rattana and Gonzalez,
                      Hervé and Broquet, Antonin and van der Linden, Peter and
                      Rose, Samuel L. and Flot, David and Balandin, Taras and
                      Gordeliy, Valentin and Lahey-Rudolph, J. Mia and Roessle,
                      Manfred and de Sanctis, Daniele and Leonard, Gordon A. and
                      Mueller-Dieckmann, Christoph and Royant, Antoine},
      title        = {{T}he {TR}- ic {OS} setup at the {ESRF}: time-resolved
                      microsecond {UV}–{V}is absorption spectroscopy on protein
                      crystals},
      journal      = {Acta crystallographica / Section D},
      volume       = {80},
      number       = {1},
      issn         = {0907-4449},
      address      = {Bognor Regis},
      publisher    = {Wiley},
      reportid     = {FZJ-2024-03243},
      pages        = {16 - 25},
      year         = {2024},
      abstract     = {The technique of time-resolved macromolecular
                      crystallography (TR-MX) has recently been rejuvenated at
                      synchrotrons, resulting in the design of dedicated
                      beamlines. Using pump-probe schemes, this should make the
                      mechanistic study of photoactive proteins and other suitable
                      systems possible with time resolutions down to microseconds.
                      In order to identify relevant time delays, time-resolved
                      spectroscopic experiments directly performed on protein
                      crystals are often desirable. To this end, an instrument has
                      been built at the icOS Lab (in crystallo Optical
                      Spectroscopy Laboratory) at the European Synchrotron
                      Radiation Facility using reflective focusing objectives with
                      a tuneable nanosecond laser as a pump and a microsecond
                      xenon flash lamp as a probe, called the TR-icOS
                      (time-resolved icOS) setup. Using this instrument,
                      pump-probe spectra can rapidly be recorded from single
                      crystals with time delays ranging from a few microseconds to
                      seconds and beyond. This can be repeated at various laser
                      pulse energies to track the potential presence of artefacts
                      arising from two-photon absorption, which amounts to a power
                      titration of a photoreaction. This approach has been applied
                      to monitor the rise and decay of the M state in the
                      photocycle of crystallized bacteriorhodopsin and showed that
                      the photocycle is increasingly altered with laser pulses of
                      peak fluence greater than 100 mJ cm-2, providing
                      experimental laser and delay parameters for a successful
                      TR-MX experiment.},
      cin          = {IBI-7},
      ddc          = {530},
      cid          = {I:(DE-Juel1)IBI-7-20200312},
      pnm          = {5241 - Molecular Information Processing in Cellular Systems
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {38088897},
      UT           = {WOS:001168282500003},
      doi          = {10.1107/S2059798323010483},
      url          = {https://juser.fz-juelich.de/record/1025965},
}