Home > Publications database > The TR- ic OS setup at the ESRF: time-resolved microsecond UV–Vis absorption spectroscopy on protein crystals > print |
001 | 1025965 | ||
005 | 20250204113851.0 | ||
024 | 7 | _ | |a 10.1107/S2059798323010483 |2 doi |
024 | 7 | _ | |a 0907-4449 |2 ISSN |
024 | 7 | _ | |a 1399-0047 |2 ISSN |
024 | 7 | _ | |a 2059-7983 |2 ISSN |
024 | 7 | _ | |a 10.34734/FZJ-2024-03243 |2 datacite_doi |
024 | 7 | _ | |a 38088897 |2 pmid |
024 | 7 | _ | |a WOS:001168282500003 |2 WOS |
037 | _ | _ | |a FZJ-2024-03243 |
082 | _ | _ | |a 530 |
100 | 1 | _ | |a Engilberge, Sylvain |0 0000-0001-8680-6790 |b 0 |
245 | _ | _ | |a The TR- ic OS setup at the ESRF: time-resolved microsecond UV–Vis absorption spectroscopy on protein crystals |
260 | _ | _ | |a Bognor Regis |c 2024 |b Wiley |
336 | 7 | _ | |a article |2 DRIVER |
336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1714151643_32242 |2 PUB:(DE-HGF) |
336 | 7 | _ | |a ARTICLE |2 BibTeX |
336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
520 | _ | _ | |a The technique of time-resolved macromolecular crystallography (TR-MX) has recently been rejuvenated at synchrotrons, resulting in the design of dedicated beamlines. Using pump-probe schemes, this should make the mechanistic study of photoactive proteins and other suitable systems possible with time resolutions down to microseconds. In order to identify relevant time delays, time-resolved spectroscopic experiments directly performed on protein crystals are often desirable. To this end, an instrument has been built at the icOS Lab (in crystallo Optical Spectroscopy Laboratory) at the European Synchrotron Radiation Facility using reflective focusing objectives with a tuneable nanosecond laser as a pump and a microsecond xenon flash lamp as a probe, called the TR-icOS (time-resolved icOS) setup. Using this instrument, pump-probe spectra can rapidly be recorded from single crystals with time delays ranging from a few microseconds to seconds and beyond. This can be repeated at various laser pulse energies to track the potential presence of artefacts arising from two-photon absorption, which amounts to a power titration of a photoreaction. This approach has been applied to monitor the rise and decay of the M state in the photocycle of crystallized bacteriorhodopsin and showed that the photocycle is increasingly altered with laser pulses of peak fluence greater than 100 mJ cm-2, providing experimental laser and delay parameters for a successful TR-MX experiment. |
536 | _ | _ | |a 5241 - Molecular Information Processing in Cellular Systems (POF4-524) |0 G:(DE-HGF)POF4-5241 |c POF4-524 |f POF IV |x 0 |
588 | _ | _ | |a Dataset connected to CrossRef, Journals: juser.fz-juelich.de |
700 | 1 | _ | |a Caramello, Nicolas |0 0000-0003-0025-0213 |b 1 |
700 | 1 | _ | |a Bukhdruker, Sergei |0 0000-0002-0157-532X |b 2 |
700 | 1 | _ | |a Byrdin, Martin |0 0000-0002-6389-8714 |b 3 |
700 | 1 | _ | |a Giraud, Thierry |0 P:(DE-HGF)0 |b 4 |
700 | 1 | _ | |a Jacquet, Philippe |0 P:(DE-HGF)0 |b 5 |
700 | 1 | _ | |a Scortani, Damien |0 P:(DE-HGF)0 |b 6 |
700 | 1 | _ | |a Biv, Rattana |0 P:(DE-HGF)0 |b 7 |
700 | 1 | _ | |a Gonzalez, Hervé |0 P:(DE-HGF)0 |b 8 |
700 | 1 | _ | |a Broquet, Antonin |0 P:(DE-HGF)0 |b 9 |
700 | 1 | _ | |a van der Linden, Peter |0 0000-0002-3481-0787 |b 10 |
700 | 1 | _ | |a Rose, Samuel L. |0 0000-0003-4824-4066 |b 11 |
700 | 1 | _ | |a Flot, David |0 0000-0001-6639-7205 |b 12 |
700 | 1 | _ | |a Balandin, Taras |0 P:(DE-Juel1)131949 |b 13 |u fzj |
700 | 1 | _ | |a Gordeliy, Valentin |0 P:(DE-Juel1)131964 |b 14 |
700 | 1 | _ | |a Lahey-Rudolph, J. Mia |0 0000-0001-5279-7267 |b 15 |
700 | 1 | _ | |a Roessle, Manfred |0 P:(DE-HGF)0 |b 16 |
700 | 1 | _ | |a de Sanctis, Daniele |0 0000-0003-0391-8290 |b 17 |
700 | 1 | _ | |a Leonard, Gordon A. |0 0000-0001-5030-0122 |b 18 |
700 | 1 | _ | |a Mueller-Dieckmann, Christoph |0 0000-0003-3625-2747 |b 19 |
700 | 1 | _ | |a Royant, Antoine |0 0000-0002-1919-8649 |b 20 |
773 | _ | _ | |a 10.1107/S2059798323010483 |g Vol. 80, no. 1, p. 16 - 25 |0 PERI:(DE-600)2968623-4 |n 1 |p 16 - 25 |t Acta crystallographica / Section D |v 80 |y 2024 |x 0907-4449 |
856 | 4 | _ | |y OpenAccess |u https://juser.fz-juelich.de/record/1025965/files/qi5005.pdf |
856 | 4 | _ | |y OpenAccess |x icon |u https://juser.fz-juelich.de/record/1025965/files/qi5005.gif?subformat=icon |
856 | 4 | _ | |y OpenAccess |x icon-1440 |u https://juser.fz-juelich.de/record/1025965/files/qi5005.jpg?subformat=icon-1440 |
856 | 4 | _ | |y OpenAccess |x icon-180 |u https://juser.fz-juelich.de/record/1025965/files/qi5005.jpg?subformat=icon-180 |
856 | 4 | _ | |y OpenAccess |x icon-640 |u https://juser.fz-juelich.de/record/1025965/files/qi5005.jpg?subformat=icon-640 |
909 | C | O | |o oai:juser.fz-juelich.de:1025965 |p openaire |p open_access |p VDB |p driver |p dnbdelivery |
910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 13 |6 P:(DE-Juel1)131949 |
910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 14 |6 P:(DE-Juel1)131964 |
913 | 1 | _ | |a DE-HGF |b Key Technologies |l Natural, Artificial and Cognitive Information Processing |1 G:(DE-HGF)POF4-520 |0 G:(DE-HGF)POF4-524 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-500 |4 G:(DE-HGF)POF |v Molecular and Cellular Information Processing |9 G:(DE-HGF)POF4-5241 |x 0 |
914 | 1 | _ | |y 2024 |
915 | _ | _ | |a Creative Commons Attribution CC BY 4.0 |0 LIC:(DE-HGF)CCBY4 |2 HGFVOC |
915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0160 |2 StatID |b Essential Science Indicators |d 2023-08-23 |
915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1190 |2 StatID |b Biological Abstracts |d 2023-08-23 |
915 | _ | _ | |a DEAL Wiley |0 StatID:(DE-HGF)3001 |2 StatID |d 2023-08-23 |w ger |
915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0113 |2 StatID |b Science Citation Index Expanded |d 2023-08-23 |
915 | _ | _ | |a OpenAccess |0 StatID:(DE-HGF)0510 |2 StatID |
915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |d 2024-12-17 |
915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |d 2024-12-17 |
915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0600 |2 StatID |b Ebsco Academic Search |d 2024-12-17 |
915 | _ | _ | |a Peer Review |0 StatID:(DE-HGF)0030 |2 StatID |b ASC |d 2024-12-17 |
915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b ACTA CRYSTALLOGR D : 2022 |d 2024-12-17 |
915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Clarivate Analytics Master Journal List |d 2024-12-17 |
915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1050 |2 StatID |b BIOSIS Previews |d 2024-12-17 |
915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1030 |2 StatID |b Current Contents - Life Sciences |d 2024-12-17 |
915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |d 2024-12-17 |
915 | _ | _ | |a IF < 5 |0 StatID:(DE-HGF)9900 |2 StatID |d 2024-12-17 |
920 | _ | _ | |l yes |
920 | 1 | _ | |0 I:(DE-Juel1)IBI-7-20200312 |k IBI-7 |l Strukturbiochemie |x 0 |
980 | _ | _ | |a journal |
980 | _ | _ | |a VDB |
980 | _ | _ | |a UNRESTRICTED |
980 | _ | _ | |a I:(DE-Juel1)IBI-7-20200312 |
980 | 1 | _ | |a FullTexts |
Library | Collection | CLSMajor | CLSMinor | Language | Author |
---|