001     1025965
005     20250204113851.0
024 7 _ |a 10.1107/S2059798323010483
|2 doi
024 7 _ |a 0907-4449
|2 ISSN
024 7 _ |a 1399-0047
|2 ISSN
024 7 _ |a 2059-7983
|2 ISSN
024 7 _ |a 10.34734/FZJ-2024-03243
|2 datacite_doi
024 7 _ |a 38088897
|2 pmid
024 7 _ |a WOS:001168282500003
|2 WOS
037 _ _ |a FZJ-2024-03243
082 _ _ |a 530
100 1 _ |a Engilberge, Sylvain
|0 0000-0001-8680-6790
|b 0
245 _ _ |a The TR- ic OS setup at the ESRF: time-resolved microsecond UV–Vis absorption spectroscopy on protein crystals
260 _ _ |a Bognor Regis
|c 2024
|b Wiley
336 7 _ |a article
|2 DRIVER
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|b journal
|m journal
|0 PUB:(DE-HGF)16
|s 1714151643_32242
|2 PUB:(DE-HGF)
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a Journal Article
|0 0
|2 EndNote
520 _ _ |a The technique of time-resolved macromolecular crystallography (TR-MX) has recently been rejuvenated at synchrotrons, resulting in the design of dedicated beamlines. Using pump-probe schemes, this should make the mechanistic study of photoactive proteins and other suitable systems possible with time resolutions down to microseconds. In order to identify relevant time delays, time-resolved spectroscopic experiments directly performed on protein crystals are often desirable. To this end, an instrument has been built at the icOS Lab (in crystallo Optical Spectroscopy Laboratory) at the European Synchrotron Radiation Facility using reflective focusing objectives with a tuneable nanosecond laser as a pump and a microsecond xenon flash lamp as a probe, called the TR-icOS (time-resolved icOS) setup. Using this instrument, pump-probe spectra can rapidly be recorded from single crystals with time delays ranging from a few microseconds to seconds and beyond. This can be repeated at various laser pulse energies to track the potential presence of artefacts arising from two-photon absorption, which amounts to a power titration of a photoreaction. This approach has been applied to monitor the rise and decay of the M state in the photocycle of crystallized bacteriorhodopsin and showed that the photocycle is increasingly altered with laser pulses of peak fluence greater than 100 mJ cm-2, providing experimental laser and delay parameters for a successful TR-MX experiment.
536 _ _ |a 5241 - Molecular Information Processing in Cellular Systems (POF4-524)
|0 G:(DE-HGF)POF4-5241
|c POF4-524
|f POF IV
|x 0
588 _ _ |a Dataset connected to CrossRef, Journals: juser.fz-juelich.de
700 1 _ |a Caramello, Nicolas
|0 0000-0003-0025-0213
|b 1
700 1 _ |a Bukhdruker, Sergei
|0 0000-0002-0157-532X
|b 2
700 1 _ |a Byrdin, Martin
|0 0000-0002-6389-8714
|b 3
700 1 _ |a Giraud, Thierry
|0 P:(DE-HGF)0
|b 4
700 1 _ |a Jacquet, Philippe
|0 P:(DE-HGF)0
|b 5
700 1 _ |a Scortani, Damien
|0 P:(DE-HGF)0
|b 6
700 1 _ |a Biv, Rattana
|0 P:(DE-HGF)0
|b 7
700 1 _ |a Gonzalez, Hervé
|0 P:(DE-HGF)0
|b 8
700 1 _ |a Broquet, Antonin
|0 P:(DE-HGF)0
|b 9
700 1 _ |a van der Linden, Peter
|0 0000-0002-3481-0787
|b 10
700 1 _ |a Rose, Samuel L.
|0 0000-0003-4824-4066
|b 11
700 1 _ |a Flot, David
|0 0000-0001-6639-7205
|b 12
700 1 _ |a Balandin, Taras
|0 P:(DE-Juel1)131949
|b 13
|u fzj
700 1 _ |a Gordeliy, Valentin
|0 P:(DE-Juel1)131964
|b 14
700 1 _ |a Lahey-Rudolph, J. Mia
|0 0000-0001-5279-7267
|b 15
700 1 _ |a Roessle, Manfred
|0 P:(DE-HGF)0
|b 16
700 1 _ |a de Sanctis, Daniele
|0 0000-0003-0391-8290
|b 17
700 1 _ |a Leonard, Gordon A.
|0 0000-0001-5030-0122
|b 18
700 1 _ |a Mueller-Dieckmann, Christoph
|0 0000-0003-3625-2747
|b 19
700 1 _ |a Royant, Antoine
|0 0000-0002-1919-8649
|b 20
773 _ _ |a 10.1107/S2059798323010483
|g Vol. 80, no. 1, p. 16 - 25
|0 PERI:(DE-600)2968623-4
|n 1
|p 16 - 25
|t Acta crystallographica / Section D
|v 80
|y 2024
|x 0907-4449
856 4 _ |y OpenAccess
|u https://juser.fz-juelich.de/record/1025965/files/qi5005.pdf
856 4 _ |y OpenAccess
|x icon
|u https://juser.fz-juelich.de/record/1025965/files/qi5005.gif?subformat=icon
856 4 _ |y OpenAccess
|x icon-1440
|u https://juser.fz-juelich.de/record/1025965/files/qi5005.jpg?subformat=icon-1440
856 4 _ |y OpenAccess
|x icon-180
|u https://juser.fz-juelich.de/record/1025965/files/qi5005.jpg?subformat=icon-180
856 4 _ |y OpenAccess
|x icon-640
|u https://juser.fz-juelich.de/record/1025965/files/qi5005.jpg?subformat=icon-640
909 C O |o oai:juser.fz-juelich.de:1025965
|p openaire
|p open_access
|p VDB
|p driver
|p dnbdelivery
910 1 _ |a Forschungszentrum Jülich
|0 I:(DE-588b)5008462-8
|k FZJ
|b 13
|6 P:(DE-Juel1)131949
910 1 _ |a Forschungszentrum Jülich
|0 I:(DE-588b)5008462-8
|k FZJ
|b 14
|6 P:(DE-Juel1)131964
913 1 _ |a DE-HGF
|b Key Technologies
|l Natural, Artificial and Cognitive Information Processing
|1 G:(DE-HGF)POF4-520
|0 G:(DE-HGF)POF4-524
|3 G:(DE-HGF)POF4
|2 G:(DE-HGF)POF4-500
|4 G:(DE-HGF)POF
|v Molecular and Cellular Information Processing
|9 G:(DE-HGF)POF4-5241
|x 0
914 1 _ |y 2024
915 _ _ |a Creative Commons Attribution CC BY 4.0
|0 LIC:(DE-HGF)CCBY4
|2 HGFVOC
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0160
|2 StatID
|b Essential Science Indicators
|d 2023-08-23
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1190
|2 StatID
|b Biological Abstracts
|d 2023-08-23
915 _ _ |a DEAL Wiley
|0 StatID:(DE-HGF)3001
|2 StatID
|d 2023-08-23
|w ger
915 _ _ |a WoS
|0 StatID:(DE-HGF)0113
|2 StatID
|b Science Citation Index Expanded
|d 2023-08-23
915 _ _ |a OpenAccess
|0 StatID:(DE-HGF)0510
|2 StatID
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0200
|2 StatID
|b SCOPUS
|d 2024-12-17
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0300
|2 StatID
|b Medline
|d 2024-12-17
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0600
|2 StatID
|b Ebsco Academic Search
|d 2024-12-17
915 _ _ |a Peer Review
|0 StatID:(DE-HGF)0030
|2 StatID
|b ASC
|d 2024-12-17
915 _ _ |a JCR
|0 StatID:(DE-HGF)0100
|2 StatID
|b ACTA CRYSTALLOGR D : 2022
|d 2024-12-17
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0199
|2 StatID
|b Clarivate Analytics Master Journal List
|d 2024-12-17
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1050
|2 StatID
|b BIOSIS Previews
|d 2024-12-17
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1030
|2 StatID
|b Current Contents - Life Sciences
|d 2024-12-17
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0150
|2 StatID
|b Web of Science Core Collection
|d 2024-12-17
915 _ _ |a IF < 5
|0 StatID:(DE-HGF)9900
|2 StatID
|d 2024-12-17
920 _ _ |l yes
920 1 _ |0 I:(DE-Juel1)IBI-7-20200312
|k IBI-7
|l Strukturbiochemie
|x 0
980 _ _ |a journal
980 _ _ |a VDB
980 _ _ |a UNRESTRICTED
980 _ _ |a I:(DE-Juel1)IBI-7-20200312
980 1 _ |a FullTexts


LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21