TY  - JOUR
AU  - Kovalev, Kirill
AU  - Tsybrov, Fedor
AU  - Alekseev, Alexey
AU  - Shevchenko, Vitaly
AU  - Soloviov, Dmytro
AU  - Siletsky, Sergey
AU  - Bourenkov, Gleb
AU  - Agthe, Michael
AU  - Nikolova, Marina
AU  - von Stetten, David
AU  - Astashkin, Roman
AU  - Bukhdruker, Sergey
AU  - Chizhov, Igor
AU  - Royant, Antoine
AU  - Kuzmin, Alexander
AU  - Gushchin, Ivan
AU  - Rosselli, Riccardo
AU  - Rodriguez-Valera, Francisco
AU  - Ilyinskiy, Nikolay
AU  - Rogachev, Andrey
AU  - Borshchevskiy, Valentin
AU  - Schneider, Thomas R.
AU  - Bamberg, Ernst
AU  - Gordeliy, Valentin
TI  - Mechanisms of inward transmembrane proton translocation
JO  - Nature structural & molecular biology
VL  - 30
IS  - 7
SN  - 1545-9993
CY  - London [u.a.]
PB  - Nature Publishing Group
M1  - FZJ-2024-03247
SP  - 970 - 979
PY  - 2023
AB  - Proton transport is indispensable for cell life. It is believed that molecular mechanisms of proton movement through different types of proton-conducting molecules have general universal features. However, elucidation of such mechanisms is a challenge. It requires true-atomic-resolution structures of all key proton-conducting states. Here we present a comprehensive function-structure study of a light-driven bacterial inward proton pump, xenorhodopsin, from Bacillus coahuilensis in all major proton-conducting states. The structures reveal that proton translocation is based on proton wires regulated by internal gates. The wires serve as both selectivity filters and translocation pathways for protons. The cumulative results suggest a general concept of proton translocation. We demonstrate the use of serial time-resolved crystallography at a synchrotron source with sub-millisecond resolution for rhodopsin studies, opening the door for principally new applications. The results might also be of interest for optogenetics since xenorhodopsins are the only alternative tools to fire neurons.
LB  - PUB:(DE-HGF)16
C6  - 37386213
UR  - <Go to ISI:>//WOS:001020309800001
DO  - DOI:10.1038/s41594-023-01020-9
UR  - https://juser.fz-juelich.de/record/1025970
ER  -