TY  - THES
AU  - Shihada, Jamal
TI  - 3D Scaffolds with Integrated Electrodes for Neuronal Cell Culture
VL  - 103
PB  - RWTH Aachen University
VL  - Dissertation
CY  - Jülich
M1  - FZJ-2024-03894
SN  - 978-3-95806-756-1
T2  - Schriften des Forschungszentrums Jülich Reihe Information / Information
SP  - vii, 163
PY  - 2024
N1  - Dissertation, RWTH Aachen University, 2024
AB  - Three-dimensional (3D) neuronal systems like neuronal tissues and organoids have become increasingly important in neural science as, due to their 3D nature, they provide a more realistic environment of the brain and thus can mimic specific brain regionsmore precisely than two-dimensional (2D) systems. However, these 3D systems need additional structures, called scaffolds, which support the neuronal growth in all three dimensions. Recent technological advances in microfabrication can be used to improve the quality of the scaffolds, and thus of the 3D systems. To get a better understanding of the complex 3D environment within such 3D systems it is furthermore essential to monitor the electrical activities of these environments. To be able to monitor electricalsignals in all three dimensions real 3D electrodes have to be used as conventional 2D electrodes only can monitor surface activities. Therefore, this work introduces a new platform for real 3D electrodes as well as 3D scaffold systems to investigatedifferent 3D neuronal systems. First of all, hollow cylinders are printed onto different 2D substrates with the help of a two-photon polymerization 3D printer. A template-assisted electrochemical deposition process of gold is used to extend the electrodes into thethird dimension. 3D electrodes with less than 10 μm diameter and up to 150 μm height are then used to monitor spontaneous as well as light-induced electrical activities from extracted rat retina and human brain slices in different depths of the tissues. Finally,3D electrodes are integrated into a scaffold system to enable a profound analysis of precisely controlled 3D neuronal environments. The high resolution stiff scaffold system is fabricated with using a two-photon polymerization process and supports the growthof embryonic, cortical rat neurons in all three dimensions. After the network has been established electrical signals with high amplitudes were monitored in different heights within the network. This combined approach allows for a comprehensive investigationof 3D neuronal in-vitro platforms at any life cycle stage.
LB  - PUB:(DE-HGF)3 ; PUB:(DE-HGF)11
DO  - DOI:10.34734/FZJ-2024-03894
UR  - https://juser.fz-juelich.de/record/1027485
ER  -