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@ARTICLE{Jessing:1028861,
      author       = {Jessing, Max and Abuawad, Abdalhalim and Bikulov, Timur and
                      Abresch, Jan and Offenhäusser, Andreas and Krause,
                      Hans-Joachim},
      title        = {{I}sothermal {A}mplification {U}sing
                      {T}emperature-{C}ontrolled {F}requency {M}ixing {M}agnetic
                      {D}etection-{B}ased {P}ortable {F}ield-{T}esting {P}latform},
      journal      = {Sensors},
      volume       = {24},
      number       = {14},
      issn         = {1424-8220},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2024-04847},
      pages        = {4478 -},
      year         = {2024},
      abstract     = {Sensitive magnetic nucleic acid (NA) detection via
                      frequency mixing magnetic detection (FMMD) requires
                      amplified NA samples for which a reliable temperature
                      control is necessary. The feasibility of recombinase
                      polymerase amplification (RPA) was studied within a newly
                      integrated temperature-controlled sensor unit of a mobile
                      FMMD based setup. It has been demonstrated that the
                      inherently generated heat of the low frequency (LF)
                      excitation signal of FMMD can be utilized and controlled by
                      means of pulse width modulation (PWM). To test control
                      performance in a point of care (PoC) setting with changing
                      ambient conditions, a steady state and dynamic response
                      model for the thermal behavior at the sample position of the
                      sensor were developed. We confirmed that in the sensor unit
                      of the FMMD device, RPA performs similar as in a
                      temperature-controlled water bath. For narrow steady state
                      temperature regions, a linear extrapolation suffices for
                      estimation of the sample position temperature, based on the
                      temperature feedback sensor for PWM control. For any other
                      ambient conditions, we identified and validated a lumped
                      parameter model (LPM) performing with high estimation
                      accuracy. We expect that the method can be used for NA
                      amplification and magnetic detection using FMMD in
                      resource-limited settings.},
      cin          = {IBI-3},
      ddc          = {620},
      cid          = {I:(DE-Juel1)IBI-3-20200312},
      pnm          = {5241 - Molecular Information Processing in Cellular Systems
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {39065876},
      UT           = {WOS:001277300600001},
      doi          = {10.3390/s24144478},
      url          = {https://juser.fz-juelich.de/record/1028861},
}