Hauptseite > Publikationsdatenbank > Isothermal Amplification Using Temperature-Controlled Frequency Mixing Magnetic Detection-Based Portable Field-Testing Platform > print

001     1028861
005     20250204113911.0
024 7 _ |a 10.3390/s24144478
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024 7 _ |a 10.34734/FZJ-2024-04847
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082 _ _ |a 620
100 1 _ |a Jessing, Max
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245 _ _ |a Isothermal Amplification Using Temperature-Controlled Frequency Mixing Magnetic Detection-Based Portable Field-Testing Platform
260 _ _ |a Basel
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520 _ _ |a Sensitive magnetic nucleic acid (NA) detection via frequency mixing magnetic detection (FMMD) requires amplified NA samples for which a reliable temperature control is necessary. The feasibility of recombinase polymerase amplification (RPA) was studied within a newly integrated temperature-controlled sensor unit of a mobile FMMD based setup. It has been demonstrated that the inherently generated heat of the low frequency (LF) excitation signal of FMMD can be utilized and controlled by means of pulse width modulation (PWM). To test control performance in a point of care (PoC) setting with changing ambient conditions, a steady state and dynamic response model for the thermal behavior at the sample position of the sensor were developed. We confirmed that in the sensor unit of the FMMD device, RPA performs similar as in a temperature-controlled water bath. For narrow steady state temperature regions, a linear extrapolation suffices for estimation of the sample position temperature, based on the temperature feedback sensor for PWM control. For any other ambient conditions, we identified and validated a lumped parameter model (LPM) performing with high estimation accuracy. We expect that the method can be used for NA amplification and magnetic detection using FMMD in resource-limited settings.
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700 1 _ |a Abuawad, Abdalhalim
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700 1 _ |a Bikulov, Timur
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700 1 _ |a Abresch, Jan
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700 1 _ |a Offenhäusser, Andreas
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700 1 _ |a Krause, Hans-Joachim
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773 _ _ |a 10.3390/s24144478
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