TY  - JOUR
AU  - Üffing, Alina
AU  - Weiergräber, Oliver H.
AU  - Schwarten, Melanie
AU  - Hoffmann, Silke
AU  - Willbold, Dieter
TI  - GABARAP interacts with EGFR — supporting the unique role of this hAtg8 protein during receptor trafficking
JO  - FEBS letters
VL  - x
IS  - x
SN  - 0014-5793
CY  - Chichester
PB  - Wiley
M1  - FZJ-2024-05317
SP  - online ahead of print
PY  - 2024
AB  - The human Atg8 family member GABARAP is involved in numerous autophagy-related and -unrelated processes. We recently observed that specifically the deficiency of GABARAP enhances epidermal growth factor receptor (EGFR) degradation upon ligand stimulation. Here, we report on two putative LC3-interacting regions (LIRs) within EGFR, the first of which (LIR1) is selected as a GABARAP binding site in silico. Indeed, in vitro interaction studies reveal preferential binding of LIR1 to GABARAP and GABARAPL1. Our X-ray data demonstrate interaction of core LIR1 residues FLPV with both hydrophobic pockets of GABARAP suggesting canonical binding. Although LIR1 occupies the LIR docking site, GABARAP Y49 and L50 appear dispensable in this case. Our data support the hypothesis that GABARAP affects the fate of EGFR at least in part through direct binding.
LB  - PUB:(DE-HGF)16
C6  - 39160442
UR  - <Go to ISI:>//WOS:001285593800001
DO  - DOI:10.1002/1873-3468.14997
UR  - https://juser.fz-juelich.de/record/1030471
ER  -