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@ARTICLE{Junglas:1030511,
author = {Junglas, Benedikt and Gewehr, Lucas and Mernberger, Lara
and Schönnenbeck, Philipp and Jilly, Ruven and Hellmann,
Nadja and Schneider, Dirk and Sachse, Carsten},
title = {{S}tructural basis for {GTP}ase activity and conformational
changes of the bacterial dynamin-like protein {S}yn{DLP}},
journal = {Cell reports},
volume = {43},
number = {9},
issn = {2211-1247},
address = {[New York, NY]},
publisher = {Elsevier},
reportid = {FZJ-2024-05334},
pages = {114657 -},
year = {2024},
abstract = {SynDLP, a dynamin-like protein (DLP) encoded in the
cyanobacterium Synechocystis sp. PCC 6803, has recently been
identified to be structurally highly similar to eukaryotic
dynamins. To elucidate structural changes during guanosine
triphosphate (GTP) hydrolysis, we solved the cryoelectron
microscopy (cryo-EM) structures of oligomeric full-length
SynDLP after addition of guanosine diphosphate (GDP) at 4.1
Å and GTP at 3.6-Å resolution as well as a GMPPNP-bound
dimer structure of a minimal G-domain construct of SynDLP at
3.8-Å resolution. In comparison with what has been seen in
the previously resolved apo structure, we found that the
G-domain is tilted upward relative to the stalk upon GTP
hydrolysis and that the G-domain dimerizes via an additional
extended dimerization domain not present in canonical
G-domains. When incubated with lipid vesicles, we observed
formation of irregular tubular SynDLP assemblies that
interact with negatively charged lipids. Here, we provide
the structural framework of a series of different functional
SynDLP assembly states during GTP turnover.},
cin = {ER-C-3},
ddc = {610},
cid = {I:(DE-Juel1)ER-C-3-20170113},
pnm = {5352 - Understanding the Functionality of Soft Matter and
Biomolecular Systems (POF4-535) / 5241 - Molecular
Information Processing in Cellular Systems (POF4-524)},
pid = {G:(DE-HGF)POF4-5352 / G:(DE-HGF)POF4-5241},
typ = {PUB:(DE-HGF)16},
pubmed = {39207903},
UT = {WOS:001316475700001},
doi = {10.1016/j.celrep.2024.114657},
url = {https://juser.fz-juelich.de/record/1030511},
}