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@ARTICLE{Vercellino:1030701,
      author       = {Vercellino, Irene and Sottatipreedawong, Muratha and Kazmi,
                      Ahad Ali},
      title        = {{H}ow cryo-{EM} revolutionized the field of bioenergetics},
      journal      = {Microscopy and microanalysis},
      volume       = {31},
      number       = {1},
      issn         = {1079-8501},
      address      = {Oxford},
      publisher    = {Oxford University Press},
      reportid     = {FZJ-2024-05412},
      pages        = {ozae089},
      year         = {2025},
      abstract     = {Ten years ago, the term “resolution revolution” was
                      used for the first time to describe how cryogenic electron
                      microscopy (cryo-EM) marked the beginning of a new era in
                      the field of structural biology, enabling the investigation
                      of previously unsolvable protein targets. The success of
                      cryo-EM was recognized with the 2017 Chemistry Nobel Prize
                      and has become a widely used method for the structural
                      characterization of biological macromolecules, quickly
                      catching up to x-ray crystallography. Bioenergetics is the
                      division of biochemistry that studies the mechanisms of
                      energy conversion in living organisms, strongly focused on
                      the molecular machines (enzymes) that carry out these
                      processes in cells. As bioenergetic enzymes can be arranged
                      in complexes characterized by conformational
                      heterogeneity/flexibility, they represent challenging
                      targets for structural investigation by crystallography.
                      Over the last decade, cryo-EM has therefore become a
                      powerful tool to investigate the structure and function of
                      bioenergetic complexes; here, we provide an overview of the
                      main achievements enabled by the technique. We first
                      summarize the features of cryo-EM and compare them to x-ray
                      crystallography, and then, we present the exciting
                      discoveries brought about by cryo-EM, particularly but not
                      exclusively focusing on the oxidative phosphorylation
                      system, which is a crucial energy-converting mechanism in
                      humans.},
      cin          = {ER-C-3},
      ddc          = {500},
      cid          = {I:(DE-Juel1)ER-C-3-20170113},
      pnm          = {5352 - Understanding the Functionality of Soft Matter and
                      Biomolecular Systems (POF4-535) / 5241 - Molecular
                      Information Processing in Cellular Systems (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5352 / G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {39298136},
      UT           = {WOS:001315352100001},
      doi          = {10.1093/mam/ozae089},
      url          = {https://juser.fz-juelich.de/record/1030701},
}