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@INPROCEEDINGS{Metzner:1031866,
author = {Metzner, Ralf and Chlubek, Antonia and Pflugfelder, Daniel
and Huber, Gregor and Knief, Claudia and Schultes, Sina and
Yu, Peng and Koller, Robert},
title = {11{C}-labelling in combination with {P}ositron {E}mission
{T}omography ({PET}) for investigating carbon transport
dynamics in complex root systems},
reportid = {FZJ-2024-05874},
year = {2024},
abstract = {The plastic response of a plant to environmental changes
enhances its ability to avoid environmental constraints, and
hence supports growth, reproduction, and evolutionary and
agricultural success. Non-invasive imaging methods,
including Magnetic Resonance Imaging (MRI) and Computed
Tomography (CT) have allowed for major progress in
understanding root structural development. The underlying
dynamics of the carbon transport and allocation within the
root system, however, is still not understood in detail and
sometimes similar and constant flow of carbon (C) in all
roots is assumed for the lack of detailed data. Our
development of a 11C-PET facility supported by MRI aims at
routine tracking of short-lived C isotope tracer
dynamically, non-invasively and in 3D within the root
system. This allows analysing tracer flow in different roots
at the same time and over days and weeks of development. The
11C tracer is applied non-invasively to the plant leaves as
11CO2 and imaged by a dedicated PET system (‘phenoPET’)
built in-house that was installed in a climate chamber with
full environmental control. The field of view of the system
is 18 cm in diameter and 20 cm in height.The root systems of
several maize plants were analysed for tracer flow
velocities and allocation patterns. We found significant
differences in tracer flow velocity between root types and
during root growth, as well as diurnal rhythms and a strong
impact of lateral root formation. Our results highlight the
advantages of 11C--PET for non-invasively quantifying the
dynamics in C transport. The dynamics within root systems
are more complex than hitherto expected.},
month = {Jun},
date = {2024-06-02},
organization = {12th International Symposium of the
International Society of Root Research,
Leipzig (Germany), 2 Jun 2024 - 7 Jun
2024},
subtyp = {After Call},
cin = {IBG-2},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {2171 - Biological and environmental resources for
sustainable use (POF4-217)},
pid = {G:(DE-HGF)POF4-2171},
typ = {PUB:(DE-HGF)6},
url = {https://juser.fz-juelich.de/record/1031866},
}