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001032228 005__ 20241108205840.0
001032228 0247_ $$2datacite_doi$$a10.34734/FZJ-2024-06075
001032228 037__ $$aFZJ-2024-06075
001032228 041__ $$aEnglish
001032228 1001_ $$0P:(DE-Juel1)168454$$aJunker-Frohn, Laura Verena$$b0$$eCorresponding author
001032228 1112_ $$aInternational Society of Root Research 12th International Symposium$$cLeipzig$$d2024-06-02 - 2024-06-07$$g12ISRR2024$$wGermany
001032228 245__ $$aDevelopment of a standardized protocol for phenotyping of genotypic variation in sunflower root traits in response to drought stress
001032228 260__ $$c2024
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001032228 520__ $$aSunflower is a globally grown oilseed crop, but yield is increasingly affected in many growing regions by drought periods. In the EU funded project CROPINNO, we aim to identify drought tolerance traits of sunflower, to facilitate breeding of drought tolerant cultivars. In a first experiment using the novel automated GrowScreen-Rhizo III phenotyping facility with soil-filled rhizotrons, we developed a standardized protocol to study sunflower root and shoot traits of inbred lines and commercial hybrids under control and drought conditions. Control plants were grown in well-watered peat substrate and watered daily. Drought plants were grown in predried substrate, and only watered once initially to facilitate germination. Plants were imaged daily to monitor root and shoot growth development non-invasively. After two weeks, stomatal conductance (gs) was measured and biomass, leaf area and total root length were destructively quantified. We hypothesized, that the drought stress protocol allows to reliably quantify differences in root traits in response to different soil water conditions, and to identify genotypic variation under drought conditions. Predried substrate and initial watering allowed establishment of most plants, but severely hampered plant growth. Drought stress of all lines was indicated by reduced root growth rate and lower biomass and gs compared to control plants. Hybrids were very similar in all quantified root traits, but showed a much faster growth compared to inbred lines. Inbred lines varied in root traits, including a wider root system of lines known for superior drought tolerance in the field. The results indicate the suitability of the applied protocol to monitor sunflower root growth under drought conditions, and demonstrate genotypic variation in root traits. Future experiments will include more genotypes to identify genomic and epigenetic markers which will facilitate breeding of cultivars with enhanced drought tolerance.
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001032228 536__ $$0G:(EU-Grant)101059784$$aCROPINNO - STEPPING UP SCIENTIFIC EXCELLENCE AND INNOVATION CAPACITY FOR CLIMATE-RESILIENT CROP IMPROVEMENT AND PRODUCTION (101059784)$$c101059784$$fHORIZON-WIDERA-2021-ACCESS-02$$x1
001032228 7001_ $$0P:(DE-Juel1)159493$$aLenz, Henning$$b1$$ufzj
001032228 7001_ $$0P:(DE-Juel1)145055$$aGalinski, Anna$$b2$$ufzj
001032228 7001_ $$0P:(DE-HGF)0$$aDedić, Boško$$b3
001032228 7001_ $$0P:(DE-HGF)0$$aĆuk, Nemanja$$b4
001032228 7001_ $$0P:(DE-HGF)0$$aMiladinović, Dragana$$b5
001032228 7001_ $$0P:(DE-Juel1)129373$$aNagel, Kerstin$$b6$$ufzj
001032228 8564_ $$uhttps://www.12-isrr-2024.de/
001032228 8564_ $$uhttps://juser.fz-juelich.de/record/1032228/files/Poster.pdf$$yOpenAccess
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001032228 9141_ $$y2024
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