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@INPROCEEDINGS{JunkerFrohn:1032228,
      author       = {Junker-Frohn, Laura Verena and Lenz, Henning and Galinski,
                      Anna and Dedić, Boško and Ćuk, Nemanja and Miladinović,
                      Dragana and Nagel, Kerstin},
      title        = {{D}evelopment of a standardized protocol for phenotyping of
                      genotypic variation in sunflower root traits in response to
                      drought stress},
      reportid     = {FZJ-2024-06075},
      year         = {2024},
      abstract     = {Sunflower is a globally grown oilseed crop, but yield is
                      increasingly affected in many growing regions by drought
                      periods. In the EU funded project CROPINNO, we aim to
                      identify drought tolerance traits of sunflower, to
                      facilitate breeding of drought tolerant cultivars. In a
                      first experiment using the novel automated GrowScreen-Rhizo
                      III phenotyping facility with soil-filled rhizotrons, we
                      developed a standardized protocol to study sunflower root
                      and shoot traits of inbred lines and commercial hybrids
                      under control and drought conditions. Control plants were
                      grown in well-watered peat substrate and watered daily.
                      Drought plants were grown in predried substrate, and only
                      watered once initially to facilitate germination. Plants
                      were imaged daily to monitor root and shoot growth
                      development non-invasively. After two weeks, stomatal
                      conductance (gs) was measured and biomass, leaf area and
                      total root length were destructively quantified. We
                      hypothesized, that the drought stress protocol allows to
                      reliably quantify differences in root traits in response to
                      different soil water conditions, and to identify genotypic
                      variation under drought conditions. Predried substrate and
                      initial watering allowed establishment of most plants, but
                      severely hampered plant growth. Drought stress of all lines
                      was indicated by reduced root growth rate and lower biomass
                      and gs compared to control plants. Hybrids were very similar
                      in all quantified root traits, but showed a much faster
                      growth compared to inbred lines. Inbred lines varied in root
                      traits, including a wider root system of lines known for
                      superior drought tolerance in the field. The results
                      indicate the suitability of the applied protocol to monitor
                      sunflower root growth under drought conditions, and
                      demonstrate genotypic variation in root traits. Future
                      experiments will include more genotypes to identify genomic
                      and epigenetic markers which will facilitate breeding of
                      cultivars with enhanced drought tolerance.},
      month         = {Jun},
      date          = {2024-06-02},
      organization  = {International Society of Root Research
                       12th International Symposium, Leipzig
                       (Germany), 2 Jun 2024 - 7 Jun 2024},
      subtyp        = {After Call},
      cin          = {IBG-2},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217) / CROPINNO - STEPPING UP
                      SCIENTIFIC EXCELLENCE AND INNOVATION CAPACITY FOR
                      CLIMATE-RESILIENT CROP IMPROVEMENT AND PRODUCTION
                      (101059784)},
      pid          = {G:(DE-HGF)POF4-2171 / G:(EU-Grant)101059784},
      typ          = {PUB:(DE-HGF)6},
      doi          = {10.34734/FZJ-2024-06075},
      url          = {https://juser.fz-juelich.de/record/1032228},
}