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@INPROCEEDINGS{Paul:1033892,
author = {Paul, Richard D. and Seiffarth, Johannes and Scharr, Hanno
and Nöh, Katharina},
title = {{R}obust {A}pproximate {C}haracterization of
{S}ingle-{C}ell {H}eterogeneity in {M}icrobial {G}rowth},
publisher = {IEEE},
reportid = {FZJ-2024-06730},
isbn = {979-8-3503-1333-8},
pages = {1-5},
year = {2024},
abstract = {Live-cell microscopy allows to go beyond measuring average
features of cellular populations to observe, quantify and
explain biological heterogeneity. Deep Learning-based
instance segmentation and cell tracking form the gold
standard analysis tools to process the microscopy data
collected, but tracking in particular suffers severely from
low temporal resolution. In this work, we show that
approximating cell cycle time distributions in microbial
colonies of C. glutamicum is possible without performing
tracking, even at low temporal resolution. To this end, we
infer the parameters of a stochastic multi-stage birth
process model using the Bayesian Synthetic Likelihood method
at varying temporal resolutions by subsampling microscopy
sequences, for which ground truth tracking is available. Our
results indicate, that the proposed approach yields high
quality approximations even at very low temporal resolution,
where tracking fails to yield reasonable results.},
month = {May},
date = {2024-05-27},
organization = {2024 IEEE International Symposium on
Biomedical Imaging (ISBI), Athens
(Greece), 27 May 2024 - 30 May 2024},
cin = {IAS-8 / IBG-1},
cid = {I:(DE-Juel1)IAS-8-20210421 / I:(DE-Juel1)IBG-1-20101118},
pnm = {2171 - Biological and environmental resources for
sustainable use (POF4-217) / 5112 - Cross-Domain Algorithms,
Tools, Methods Labs (ATMLs) and Research Groups (POF4-511)},
pid = {G:(DE-HGF)POF4-2171 / G:(DE-HGF)POF4-5112},
typ = {PUB:(DE-HGF)8 / PUB:(DE-HGF)7},
UT = {WOS:001305705100163},
doi = {10.1109/ISBI56570.2024.10635267},
url = {https://juser.fz-juelich.de/record/1033892},
}