TY  - JOUR
AU  - Melnikov, Nataly
AU  - Junglas, Benedikt
AU  - Halbi, Gal
AU  - Nachmias, Dikla
AU  - Zerbib, Erez
AU  - Gueta, Noam
AU  - Upcher, Alexander
AU  - Zalk, Ran
AU  - Sachse, Carsten
AU  - Bernheim-Groswasser, Anne
AU  - Elia, Natalie
TI  - The Asgard archaeal ESCRT-III system forms helical filaments and remodels eukaryotic-like membranes
JO  - The EMBO journal
VL  - 44
SN  - 0261-4189
CY  - Hoboken, NJ [u.a.]
PB  - Wiley
M1  - FZJ-2025-00217
SP  - 665-681
PY  - 2025
AB  - The ESCRT machinery mediates membrane remodeling in numerous processes in cells including cell division and nuclear membrane reformation. The identification of ESCRT homologs in Asgardarchaea, currently considered the closest prokaryotic relative of eukaryotes, implies a role for ESCRTs in the membrane remodeling processes that occurred during eukaryogenesis. Yet, the function of these distant ESCRT homologs is mostly unresolved. Here we show that Asgard ESCRT-III proteins of the Lokiarcheota self-assemble into helical filaments, a hallmark of the ESCRT system. We determined the cryo-EM structure of the filaments at 3.6 Å resolution and found that they share features of bacterial and eukaryotic ESCRT-III assemblies. Markedly, Asgard ESCRT-III filaments bound and deformed eukaryotic-like membrane vesicles. Oligonucleotides facilitated the assembly of ESCRT-III filaments and tuned the extent of membrane remodeling. The ability of Asgard archaeal ESCRTs to remodel eukaryotic-like membranes, which are fundamentally different from archaeal membranes, and the structural properties of these proteins places them at the junction between prokaryotes and eukaryotes.
LB  - PUB:(DE-HGF)16
C6  - 39753954
UR  - <Go to ISI:>//WOS:001388934600001
DO  - DOI:10.1038/s44318-024-00346-4
UR  - https://juser.fz-juelich.de/record/1035128
ER  -