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@ARTICLE{Just:1037250,
author = {Just, Nathalie and Hoehn, Mathias},
title = {{T}o intubate or not? {B}alancing anesthesia in rodent
f{MRI}: strategies to mitigate confounding effects},
journal = {Cerebral cortex},
volume = {35},
number = {2},
issn = {1047-3211},
address = {Oxford},
publisher = {Oxford Univ. Press},
reportid = {FZJ-2025-00583},
pages = {bhae499},
year = {2025},
note = {This work was supported by the Lundbeck Foundation
(Experi-ment grant, grant nr. R370-2021-402) to N.J},
abstract = {More than a decade ago, the introduction of intubation and
mechanical ventilation for performing blood oxygen
level–dependent functional MRI studies in the rodent brain
allowed an improved control over the physiological
conditions during scanning sessions. An accurate
understanding of respiratory parameters permits to respect
the 3Rs in animal research, improves significantly the fMRI
outcome, and promises improved translational studies.
Developments also prompted a better comprehension on
anesthetics and their impact on rodent brain physiology and
function, bringing new insights on the buildup of carbon
dioxide, interhemispheric connectivity, or arousal, which
understanding are paramount for maturing better fMRI
protocols in awake rodents. Despite many arguments in favor
of intubation and subsequent mechanical ventilation, there
are also many valid against it. Most importantly, the choice
to intubate depends on the anesthesia protocol, where in
some cases intubation is essential and impractical in
others. This review does not advocate for one approach over
the other. Instead, by examining the literature from the
past two decades, we aim to provide a comprehensive review
of the pros and cons of intubation and mechanical
ventilation in fMRI studies, offering arguments for an
informed decision tailored to the respective research
question.},
cin = {INM-3},
ddc = {610},
cid = {I:(DE-Juel1)INM-3-20090406},
pnm = {5251 - Multilevel Brain Organization and Variability
(POF4-525)},
pid = {G:(DE-HGF)POF4-5251},
typ = {PUB:(DE-HGF)16},
doi = {10.1093/cercor/bhae499},
url = {https://juser.fz-juelich.de/record/1037250},
}