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@INPROCEEDINGS{Sanow:1037325,
      author       = {Sanow, Stefan and Macabuhay, Allene and Kapitanska, Olha
                      and Kant, Josefine and Lenz, Henning and Ehrlich, Tanja and
                      Kelm, Jana and Hernandez-Soriano, Maria and Huesgen, Pitter
                      and Nagel, Kerstin and Roessner, Ute and Watt, Michelle and
                      Arsova, Borjana},
      title        = {{S}tudying plant-microbe interactions for improved plant
                      abiotic resistance, insystems of progressive complexity},
      reportid     = {FZJ-2025-00644},
      pages        = {250},
      year         = {2024},
      abstract     = {Plant roots exist in a mutual interaction with the soil and
                      organisms therein. To study root interactions among abiotic
                      conditions, time and biotic factors we need to utilize
                      various experimental systems. Non-invasive phenotyping
                      quantifies growth parameters without affecting the observed
                      plants. It facilitates studies ofgrowth rates and
                      development of root system architecture (RSA) and links
                      these to underlying molecular changes (phenomics). To do so
                      we use several platforms of increasing complexity to address
                      global challenges. Tackling the mandated reduction of N
                      fertilizer, we show that Pseudomonas sp. in a gnotobiotic
                      system, canhelp Brachypodium in N deficient conditions by
                      changing RSA, supplying N, and adjusting protein and lipid
                      abundances in inoculated plants after 3 weeks (Sanow et al.,
                      2023, $\&$ unpublished). In collaboration with the Julich
                      Plant Phenotyping Center, we make standard operating
                      protocols usable with microbes. The effect oftemperature
                      increase on RSA was investigated using the semi-open Grow
                      Screen Agar system (Macabuhay et al., 2022) and showed that
                      Parabulkoholderia sp. can mitigate the stress effects by
                      changing root morphology, distribution, and increased growth
                      rates. Algae as alternative fertilizers produce distinct
                      root phenotype in Brachypodium in EcoFABs, and algal P is
                      taken up (Mau et al., 2022). An ongoing study in wheat and
                      soil-filled rhizotrons indicates that waste-water algal N is
                      also available but nutrient uptake by the algae itself must
                      be considered. Phenotypic, elemental, andmolecular results
                      for wheat are available. Field work and non-invasive
                      high-throughput root phenotyping intertwine in the
                      Wish-Roots consortium tounderstand the effect of 20 wheat
                      lines on soil health including microbiome. (OK is funded by
                      Helmholtz-Initiative für Geflüchtete 2022; JK funded by
                      EJP-Soil call 1-WISH-ROOTS Förderkennzeichen: 031B1263, SS
                      and AM are funded by JUMPA)},
      month         = {Jun},
      date          = {2024-06-02},
      organization  = {International Society of Root Research
                       12th International Symposium Roots
                       $[\&$ Roads] to a sustainable future,
                       Leipzig (Germany), 2 Jun 2024 - 7 Jun
                       2024},
      cin          = {IBG-2},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217) / JUMPA - Jülich-University of
                      Melbourne Postgraduate Academy (FAQ 151018 SA)},
      pid          = {G:(DE-HGF)POF4-2171 / G:(DE-Juel-1)FAQ 151018 SA},
      typ          = {PUB:(DE-HGF)8},
      url          = {https://juser.fz-juelich.de/record/1037325},
}