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@ARTICLE{Krause:1037786,
      author       = {Krause, Sandra and Florea, Alexandru and Choi, Chang-Hoon
                      and Worthoff, Wieland A. and Heinzel, Alexander and Fischer,
                      Saskia and Burda, Nicole and Neumaier, Bernd and Shah, N.
                      Jon and Lohmann, Philipp and Mottaghy, Felix M. and Langen,
                      Karl-Josef and Stegmayr, Carina},
      title        = {{A}utoradiography of {I}ntracerebral {T}umours in the
                      {C}hick {E}mbryo {M}odel: {A} {F}easibility {S}tudy {U}sing
                      {D}ifferent {PET} {T}racers},
      journal      = {Molecular imaging $\&$ biology},
      volume       = {27},
      number       = {1},
      issn         = {1536-1632},
      address      = {Cham},
      publisher    = {Springer Nature Switzerland},
      reportid     = {FZJ-2025-00938},
      pages        = {151–162},
      year         = {2025},
      abstract     = {Purpose In addition to rodent models, the chick embryo
                      model has gained attention for radiotracer evaluation.
                      Previous studieshave investigated tumours on the
                      chorioallantoic membrane (CAM), but its value for
                      radiotracer imaging of intracerebraltumours has yet to be
                      demonstrated.Procedures Human U87 glioblastoma cells and
                      U87-IDH1 mutant glioma cells were implanted into the brains
                      of chickembryos at developmental day 5. After 12–14 days
                      of tumour growth, blood–brain-barrier integrity was
                      evaluated in vivousing MRI contrast enhancement or ex vivo
                      with Evans blue dye. The tracers
                      O-(2-[18F]fluoroethyl)-L-tyrosine ([18F]FET)(n = 5),
                      3,4-dihydroxy-6-[18F]-fluoro-L-phenylalanine ([18F]FDOPA) (n
                      = 3), or [68Ga] labelled quinoline-based small
                      moleculefibroblast activation protein inhibitor
                      ([68Ga]FAPI-46) (n = 4) were injected intravenously if solid
                      tumours were detectedwith MRI. For time-activity curves for
                      [18F]FET, additional micro PET (μPET) was performed. The
                      chick embryos weresacrificed 60 min post-injection, and
                      cryosections of the tumour-bearing brains were produced and
                      evaluated with autoradiographyand
                      immunohistochemistry.Results Intracerebral tumours were
                      produced with a $100\%$ success rate in viable chick embryos
                      at the experimental endpoint.However, $52\%$ of chick
                      embryos (n = 85) did not survive the procedure to embryonic
                      development day 20. For the evaluatedradiotracers, the
                      tumour-to-brain ratios (TBR) derived from ex vivo
                      autoradiography, as well as the tracer kinetics derivedfrom
                      μPET for intracerebral chick embryo tumours, were
                      comparable to those previously reported in rodents and
                      patients:the TBRmean for [18F]FET was 1.69 ± 0.54 (n = 5),
                      and 3.8 for one hypermetabolic tumour and < 2.0 for two
                      isometabolictumors using [18F]FDOPA, with a TBRmean of 1.92
                      ± 1,11 (n = 3). The TBRmean of [68Ga]FAPI-46 for
                      intracerebral chickembryo tumours was 19.13 ± 0.64 (n = 4).
                      An intact blood-tumour barrier was observed in one U87-MG
                      tumour (n = 5).Conclusions Radiotracer imaging of
                      intracerebral tumours in the chick embryo offers a fast
                      model for the evaluation of radiotraceruptake, accumulation,
                      and kinetics. Our results indicate a high comparability
                      between intracerebral tumour imagingin chick embryos and
                      xenograft rodent models or brain tumour patients.},
      cin          = {INM-4 / INM-11 / JARA-BRAIN / INM-5},
      ddc          = {570},
      cid          = {I:(DE-Juel1)INM-4-20090406 / I:(DE-Juel1)INM-11-20170113 /
                      I:(DE-Juel1)VDB1046 / I:(DE-Juel1)INM-5-20090406},
      pnm          = {5253 - Neuroimaging (POF4-525) / DFG project
                      G:(GEPRIS)491111487 - Open-Access-Publikationskosten / 2025
                      - 2027 / Forschungszentrum Jülich (OAPKFZJ) (491111487) /
                      DFG project G:(GEPRIS)513201378 - Entwicklung 18F-markierter
                      Positronen-Emissions-Tomographie Tracer für die
                      nicht-invasive Erfassung von Mutationen der
                      Isocitrat-Dehydrogenase (IDH) in zerebralen Gliomen
                      (513201378)},
      pid          = {G:(DE-HGF)POF4-5253 / G:(GEPRIS)491111487 /
                      G:(GEPRIS)513201378},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {39838234},
      UT           = {WOS:001402158000001},
      doi          = {10.1007/s11307-025-01983-9},
      url          = {https://juser.fz-juelich.de/record/1037786},
}