TY  - JOUR
AU  - Aurino, Elena
AU  - Mora, Leticia
AU  - Marzocchella, Antonio
AU  - Kuchendorf , Christina M.
AU  - Ackermann, Bärbel
AU  - Hayes, Maria
TI  - Functional and Bioactive Benefits of Selected Microalgal Hydrolysates Assessed In Silico and In Vitro
JO  - Marine drugs
VL  - 23
IS  - 2
SN  - 1660-3397
CY  - Basel
PB  - MDPI
M1  - FZJ-2025-00975
SP  - 53
PY  - 2025
N1  - Dear library team, the grant under which that publication was done is theNORTH-WEST EUROPE INTERREG, grant number NWE 639 as part of the IDEA+ project
AB  - BIOPEP-UWM, a peptide database, contains 5128 peptides from a myriad of resources. Five listed peptides are Angiotensin-I-converting enzyme (ACE-1; EC3.4.15.1) inhibitory peptides derived from a red alga, while two from Chlorella vulgaris have anti-cancer and antioxidative bioactivities. Herein, we describe a process combining hydrolysis with two enzymes, Alcalase and Viscozyme, and filtration to generate protein-rich, bioactive peptide-containing hydrolysates from mixed species of Chlorella sp. and Scenedesmus sp. The potential of generated algal hydrolysates to act as food ingredients was determined by assessment of their techno-functional (foaming, emulsification, solubility, water holding, and oil holding capacity) properties. Bioactive screening of hydrolysates in vitro combined with mass spectrometry (MS) and in silico predictions identified bioactive and functional hydrolysates and six novel peptides. Peptides derived from Chlorella mix have the sequences YDYIGNNPAKGGLF and YIGNNPAKGGLF with predicted anti-inflammatory (medium confidence) and umami potential. Peptides from Scenedesmus mix have sequences IEWYGPDRPKFL, RSPTGEIIFGGETM, TVQIPGGERVPFLF, and IEWYGPDRPKFLGPF with predicted anti-inflammatory, anti-diabetic, and umami attributes. Such microalgal hydrolysates could provide essential amino acids to consumers as well as tertiary health benefits to improve human global health.
LB  - PUB:(DE-HGF)16
C6  - 39997177
UR  - <Go to ISI:>//WOS:001431550200001
DO  - DOI:10.3390/md23020053
UR  - https://juser.fz-juelich.de/record/1037823
ER  -