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@ARTICLE{Schnnenbeck:1040608,
      author       = {Schönnenbeck, Philipp and Junglas, Benedikt and Sachse,
                      Carsten},
      title        = {{C}ryo{VIA}: {A}n image analysis toolkit for the
                      quantification of membrane structures from cryo-{EM}
                      micrographs},
      journal      = {Structure},
      volume       = {33},
      number       = {4},
      issn         = {0969-2126},
      address      = {London [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2025-01957},
      pages        = {808-819.e4},
      year         = {2025},
      abstract     = {Imaging of lipid structures and associated protein
                      complexes using cryoelectron microscopy (cryo-EM) is a
                      common visualization and structure determination technique.
                      The quantitative analysis of the membrane structures,
                      however, is not routine and time consuming in particular
                      when large amounts of data are involved. Here, we introduce
                      the automated image-processing software cryo-vesicle image
                      analyzer (CryoVIA) that parametrizes lipid structures of
                      large datasets from cryo-EM images. This toolkit combines
                      segmentation, structure identification with methods to
                      automatically perform a large-scale data analysis of local
                      and global membrane properties such as bilayer thickness,
                      size, and curvature including membrane shape
                      classifications. We included analyses of exemplary datasets
                      of different lipid compositions and protein-induced lipid
                      changes through an endosomal sorting complexes required for
                      transport III (ESCRT-III) membrane remodeling protein. The
                      toolkit opens new possibilities to systematically study
                      structural properties of membrane structures and their
                      modifications from cryo-EM images.},
      cin          = {ER-C-3},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ER-C-3-20170113},
      pnm          = {5352 - Understanding the Functionality of Soft Matter and
                      Biomolecular Systems (POF4-535) / 5241 - Molecular
                      Information Processing in Cellular Systems (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5352 / G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {39919734},
      UT           = {WOS:001462904400001},
      doi          = {10.1016/j.str.2025.01.013},
      url          = {https://juser.fz-juelich.de/record/1040608},
}