guest ::
| Home > Publications database > Mapping the interaction surface between CaVβ and actin and its role in calcium channel clearance > print |
| Home > Publications database > Mapping the interaction surface between CaVβ and actin and its role in calcium channel clearance > print |
| 001 | 1042352 | ||
| 005 | 20250804115219.0 | ||
| 024 | 7 | _ | |a 10.1038/s41467-025-59548-x |2 doi |
| 024 | 7 | _ | |a 10.34734/FZJ-2025-02546 |2 datacite_doi |
| 024 | 7 | _ | |a 40348749 |2 pmid |
| 024 | 7 | _ | |a WOS:001501680700005 |2 WOS |
| 037 | _ | _ | |a FZJ-2025-02546 |
| 082 | _ | _ | |a 500 |
| 100 | 1 | _ | |a Castilla, Francisco |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Mapping the interaction surface between CaVβ and actin and its role in calcium channel clearance |
| 260 | _ | _ | |a [London] |c 2025 |b Springer Nature |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1752748917_5669 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Defective ion channel turnover and clearance of damaged proteins are associated with aging and neurodegeneration. The L-type CaV1.2 voltage-gated calcium channel mediates depolarization-induced calcium signals in heart and brain. Here, we determined the interaction surface between actin and two calcium channel subunits, CaVβ2 and CaVβ4, using cross-linking mass spectrometry and protein-protein docking, and uncovered a role in replenishing conduction-defective CaV1.2 channels. Computational and in vitro mutagenesis identified hotspots in CaVβ that decreased the affinity for actin but not for CaV1.2. When coexpressed with CaV1.2, none of the tested actin-association-deficient CaVβ mutants altered the single-channel properties or the total number of channels at the cell surface. However, coexpression with the CaVβ2 hotspot mutant downregulated current amplitudes, and with a concomitant reduction in the number of functionally available channels, indicating that current inhibition resulted from a build-up of conduction silent channels. Our findings established CaVβ2-actin interaction as a key player for clearing the plasma membrane of corrupted CaV1.2 proteins to ensure the maintenance of a functional pool of channels and proper calcium signal transduction. The CaVβ-actin molecular model introduces a potentially druggable protein-protein interface to intervene CaV-mediated signaling processes. |
| 536 | _ | _ | |a 5244 - Information Processing in Neuronal Networks (POF4-524) |0 G:(DE-HGF)POF4-5244 |c POF4-524 |f POF IV |x 0 |
| 536 | _ | _ | |a DFG project G:(GEPRIS)394431587 - FOR 2795: Synapsen unter Stress: akute Veränderungen durch mangelnde Energiezufuhr an glutamatergen Synapsen (394431587) |0 G:(GEPRIS)394431587 |c 394431587 |x 1 |
| 588 | _ | _ | |a Dataset connected to CrossRef, Journals: juser.fz-juelich.de |
| 700 | 1 | _ | |a Lugo, Victor |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Miranda-Laferte, Erick |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a Jordan, Nadine |0 P:(DE-Juel1)131932 |b 3 |u fzj |
| 700 | 1 | _ | |a Huesgen, Pitter F. |0 P:(DE-Juel1)162356 |b 4 |
| 700 | 1 | _ | |a Santiago-Schübel, Beatrix |0 P:(DE-Juel1)133853 |b 5 |e Corresponding author |
| 700 | 1 | _ | |a Alfonso-Prieto, Mercedes |0 P:(DE-Juel1)169976 |b 6 |e Corresponding author |
| 700 | 1 | _ | |a Hidalgo, Patricia |0 P:(DE-Juel1)151357 |b 7 |e Corresponding author |
| 773 | _ | _ | |a 10.1038/s41467-025-59548-x |g Vol. 16, no. 1, p. 4352 |0 PERI:(DE-600)2553671-0 |n 1 |p 4352 |t Nature Communications |v 16 |y 2025 |x 2041-1723 |
| 856 | 4 | _ | |y Restricted |
| 856 | 4 | _ | |y OpenAccess |u https://juser.fz-juelich.de/record/1042352/files/Nature_Communications_Lugo_Miranda_Hidalgo_05_2025.pdf |
| 909 | C | O | |o oai:juser.fz-juelich.de:1042352 |p openaire |p open_access |p VDB |p driver |p dnbdelivery |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 0 |6 P:(DE-HGF)0 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 1 |6 P:(DE-HGF)0 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 2 |6 P:(DE-HGF)0 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 3 |6 P:(DE-Juel1)131932 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 5 |6 P:(DE-Juel1)133853 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 6 |6 P:(DE-Juel1)169976 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 7 |6 P:(DE-Juel1)151357 |
| 913 | 1 | _ | |a DE-HGF |b Key Technologies |l Natural, Artificial and Cognitive Information Processing |1 G:(DE-HGF)POF4-520 |0 G:(DE-HGF)POF4-524 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-500 |4 G:(DE-HGF)POF |v Molecular and Cellular Information Processing |9 G:(DE-HGF)POF4-5244 |x 0 |
| 914 | 1 | _ | |y 2025 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |d 2025-01-02 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0160 |2 StatID |b Essential Science Indicators |d 2025-01-02 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1050 |2 StatID |b BIOSIS Previews |d 2025-01-02 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1190 |2 StatID |b Biological Abstracts |d 2025-01-02 |
| 915 | _ | _ | |a OpenAccess |0 StatID:(DE-HGF)0510 |2 StatID |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1040 |2 StatID |b Zoological Record |d 2025-01-02 |
| 915 | _ | _ | |a IF >= 15 |0 StatID:(DE-HGF)9915 |2 StatID |b NAT COMMUN : 2022 |d 2025-01-02 |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b NAT COMMUN : 2022 |d 2025-01-02 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0501 |2 StatID |b DOAJ Seal |d 2024-01-30T07:48:07Z |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0500 |2 StatID |b DOAJ |d 2024-01-30T07:48:07Z |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1030 |2 StatID |b Current Contents - Life Sciences |d 2025-01-02 |
| 915 | _ | _ | |a Fees |0 StatID:(DE-HGF)0700 |2 StatID |d 2025-01-02 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |d 2025-01-02 |
| 915 | _ | _ | |a Creative Commons Attribution CC BY 4.0 |0 LIC:(DE-HGF)CCBY4 |2 HGFVOC |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0113 |2 StatID |b Science Citation Index Expanded |d 2025-01-02 |
| 915 | _ | _ | |a Peer Review |0 StatID:(DE-HGF)0030 |2 StatID |b DOAJ : Peer review |d 2024-01-30T07:48:07Z |
| 915 | _ | _ | |a Article Processing Charges |0 StatID:(DE-HGF)0561 |2 StatID |d 2025-01-02 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1060 |2 StatID |b Current Contents - Agriculture, Biology and Environmental Sciences |d 2025-01-02 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |d 2025-01-02 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1150 |2 StatID |b Current Contents - Physical, Chemical and Earth Sciences |d 2025-01-02 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Clarivate Analytics Master Journal List |d 2025-01-02 |
| 920 | _ | _ | |l yes |
| 920 | 1 | _ | |0 I:(DE-Juel1)IBI-1-20200312 |k IBI-1 |l Molekular- und Zellphysiologie |x 0 |
| 920 | 1 | _ | |0 I:(DE-Juel1)IBI-7-20200312 |k IBI-7 |l Strukturbiochemie |x 1 |
| 920 | 1 | _ | |0 I:(DE-Juel1)INM-9-20140121 |k INM-9 |l Computational Biomedicine |x 2 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | _ | _ | |a I:(DE-Juel1)IBI-1-20200312 |
| 980 | _ | _ | |a I:(DE-Juel1)IBI-7-20200312 |
| 980 | _ | _ | |a I:(DE-Juel1)INM-9-20140121 |
| 980 | 1 | _ | |a FullTexts |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|