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@MISC{Kuckertz:1043717,
author = {Kuckertz, Anika and Palomero-Gallagher, Nicola and Funck,
Thomas},
title = {{A}tlas of muscarinic {M}2 receptor distributions in the
rat brain (v2)},
publisher = {EBRAINS},
reportid = {FZJ-2025-03003},
year = {2025},
abstract = {Recent technological and methodological advances have
facilitated the implementation and standardization of
functional magnetic resonance imaging (fMRI) studies in
rodents. Integration of such fMRI data with maps coding for
the cyto- and receptor architectonic organization of the
brain, as well as of its structural connectivity patterns,
will facilitate the advance of our understanding of the
biological underpinnings of functional networks and
accelerate translational research using rat models. This
dataset provides a high-resolution three-dimensional (3D)
reconstruction of receptor autoradiographs coding for the
distribution of the cholinergic muscarinic M2 receptor
throughout the entire rat brain. The autoradiographs were
obtained by means of in vitro receptor autoradiography using
the specific agonist [3H]oxotremorine-M and digitization of
the ensuing autoradiographs to enable their densitometric
analysis. The pipeline BrainBuilder, developed for the 3D
reconstruction of 2D multimodal histological datasets, was
used for the reconstruction. The resulting 3D volume was
registered to the Waxholm Space rat brain atlas (version 4),
thus enabling its integration with fMRI data.},
keywords = {Neuroscience (Other)},
cin = {INM-1},
cid = {I:(DE-Juel1)INM-1-20090406},
pnm = {5254 - Neuroscientific Data Analytics and AI (POF4-525) /
HBP SGA3 - Human Brain Project Specific Grant Agreement 3
(945539) / HIBALL - Helmholtz International BigBrain
Analytics and Learning Laboratory (HIBALL) (InterLabs-0015)
/ EBRAINS 2.0 - EBRAINS 2.0: A Research Infrastructure to
Advance Neuroscience and Brain Health (101147319)},
pid = {G:(DE-HGF)POF4-5254 / G:(EU-Grant)945539 /
G:(DE-HGF)InterLabs-0015 / G:(EU-Grant)101147319},
typ = {PUB:(DE-HGF)32},
doi = {10.25493/GF7X-2AU},
url = {https://juser.fz-juelich.de/record/1043717},
}