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| Hauptseite > Publikationsdatenbank > Guanidinylation of the cold shock protein YB ‐1: Molecular basis, structural changes and Notch‐3 receptor binding > print |
| Hauptseite > Publikationsdatenbank > Guanidinylation of the cold shock protein YB ‐1: Molecular basis, structural changes and Notch‐3 receptor binding > print |
| 001 | 1043722 | ||
| 005 | 20250820202246.0 | ||
| 024 | 7 | _ | |a 10.1002/pro.70188 |2 doi |
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| 037 | _ | _ | |a FZJ-2025-03008 |
| 082 | _ | _ | |a 610 |
| 100 | 1 | _ | |a Leitz, Anna |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Guanidinylation of the cold shock protein YB ‐1: Molecular basis, structural changes and Notch‐3 receptor binding |
| 260 | _ | _ | |a Hoboken, NJ |c 2025 |b Wiley |
| 336 | 7 | _ | |a article |2 DRIVER |
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| 520 | _ | _ | |a Posttranslational modifications of Y-box binding protein (YB)-1 are the prerequisite for its very different protein functions. Here, we investigate the underlying molecular mechanisms of YB-1 guanidinylation and link increased serum urea levels as well as the activity of glycine amidinotransferase (GATM) with guanidinylation. Computer simulations show changes in stability and conformation of the YB-1 protein induced by these modifications. In particular, the secondary structure of the doubly guanidinylated YB-1 (YB-1-2G) shows a reduced tendency to form β-sheets, and the modified cold shock domain is more exposed to the solvent. Protein-protein docking techniques in conjunction with molecular dynamics simulations confirm the binding between YB-1 and its receptor Notch-3 at EGF domains 17-24 but show no significant differences in the binding behavior of YB-1 and YB-1-2G. This is confirmed in two different types of receptor-ligand binding assays. In addition, we demonstrate for the first time a high-affinity binding of YB-1 to another ligand binding site on the Notch-3 receptor, thereby achieving effective displacement of the canonical ligand Jagged. In conclusion, we identified molecular processes that lead to the guanidinylation of YB-1 and revealed their effects on the structure and binding to receptor Notch-3. |
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| 700 | 1 | _ | |a Strodel, Birgit |0 P:(DE-Juel1)132024 |b 12 |e Corresponding author |
| 700 | 1 | _ | |a Raffetseder, Ute |0 0000-0002-7236-9793 |b 13 |e Corresponding author |
| 773 | _ | _ | |a 10.1002/pro.70188 |g Vol. 34, no. 7, p. e70188 |0 PERI:(DE-600)2000025-X |n 7 |p e70188 |t Protein science |v 34 |y 2025 |x 0961-8368 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/1043722/files/Protein%20Science%20-%202025%20-%20Leitz%20-%20Guanidinylation%20of%20the%20cold%20shock%20protein%20YB%E2%80%901%20Molecular%20basis%20structural%20changes%20and.pdf |y OpenAccess |
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