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@PHDTHESIS{Abuawad:1044576,
      author       = {Abuawad, Abdalhalim},
      title        = {{D}evelopment of {S}uperparamagnetic {B}ased {B}iological
                      {S}ensor for the {D}etection of {B}rucella {DNA} {U}sing
                      {F}requency {M}ixing {M}agnetic {D}etection},
      volume       = {113},
      school       = {RWTH Aachen University},
      type         = {Dissertation},
      address      = {Jülich},
      publisher    = {Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag},
      reportid     = {FZJ-2025-03256},
      isbn         = {978-3-95806-836-0},
      series       = {Schriften des Forschungszentrums Jülich Reihe Information
                      / Information},
      pages        = {X, 129},
      year         = {2025},
      note         = {Dissertation, RWTH Aachen University, 2025},
      abstract     = {Early detection of zoonotic diseases is essential in
                      preventing the consequences of outbreaks and reemergence
                      occurrences. Brucellosis is endemic in several countries and
                      has re-emerged with a high prevalence rate in different
                      locations, affecting livestock and public health sectors.
                      Due to the limitations of conventional Brucella detection
                      methods, including limited specificity, long incubation
                      times and safety concerns, developing a rapid, selective and
                      accurate technique for the early detection of Brucella in
                      livestock animals is crucial to prevent the spread of the
                      associated disease. In the present thesis, we introduce a
                      magnetic nanoparticle marker-based biosensor using Frequency
                      Mixing Magnetic Detection (FMMD) for the detection and
                      quantification of Brucella DNA. Magnetic nanoparticles
                      (MNPs) were used as magnetically measured markers to
                      selectively detect the target DNA hybridized with its
                      complementary capture probes immobilized on a porous
                      polyethylene filter. Our sensor demonstrated a relatively
                      fast detection time of approximately 10 min, with a
                      detection limit of 0.09 fM when tested using DNA amplified
                      from Brucella genetic material by means of Polymerase Chain
                      Reaction (PCR). In addition, the detection specificity was
                      examined using gDNA from Brucella and other zoonotic
                      bacteria that may coexist in the same niche, confirming the
                      method’s selectivity for Brucella DNA. To enhance the
                      practicality of the developed assay, we combined it with
                      isothermal Recombinase Polymerase Amplification (RPA) and
                      achieved rapid detection of 9 fM Brucella DNA in 25 minutes
                      total assay time. In addition to isothermal DNA
                      amplification in a water bath, we showed the feasibility of
                      RPA directly inside our portable FMMD-based device. When
                      being controlled by means of pulse width modulation (PWM),
                      the inherently generated heat of the low frequency (LF)
                      excitation coil of the magnetic reader can be utilized to
                      serve as a constant temperature bath for RPA, thus enabling
                      isothermal amplification inside the magnetic measurement
                      head. We confirmed that RPA performs with high efficiency in
                      the sensor unit of the FMMD device. In summary, the
                      portability of the measurement device, the selective sensing
                      of MNPs, the fast detection time, and the ability to deliver
                      quantitative results make this biosensor a valuable tool for
                      early on-site diagnosis and monitoring of Brucella
                      infections in resource-limited settings.},
      cin          = {IBI-3},
      cid          = {I:(DE-Juel1)IBI-3-20200312},
      pnm          = {5241 - Molecular Information Processing in Cellular Systems
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)3 / PUB:(DE-HGF)11},
      urn          = {urn:nbn:de:0001-2508151006385.053377491913},
      doi          = {10.34734/FZJ-2025-03256},
      url          = {https://juser.fz-juelich.de/record/1044576},
}