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@ARTICLE{Shahbaz:1044971,
      author       = {Shahbaz, Umar and Videau, Pierre and Coulonnier, Emma and
                      Papon, Carla and Navarro-Payá, David and Valenzuela, Alvaro
                      Vidal and Matus, José Tomás and Malnoy, Mickael and Zekri,
                      Olivier and Fiorani, Fabio and Faralli, Michele and Dalla
                      Costa, Lorenza},
      title        = {{R}educed stomatal density improves water-use efficiency in
                      grapevine under climate scenarios of decreased water
                      availability},
      journal      = {Plant cell reports},
      volume       = {44},
      number       = {9},
      issn         = {0721-7714},
      address      = {Heidelberg [u.a.]},
      publisher    = {Springer},
      reportid     = {FZJ-2025-03461},
      pages        = {195},
      year         = {2025},
      abstract     = {In Arabidopsis stomatal initiation relies on the
                      transcription factor SPEECHLESS, which is positively
                      regulated by AtEPFL9, a peptide of the epidermal patterning
                      factor family. In grapevine, two EPFL9 paralogs exist but
                      despite a structural similarity, their specific function
                      remains unclear. In this study, we investigated their
                      distinct functional roles and the extent to which reduced
                      stomatal density (SD) may be beneficial for grapevine in
                      terms of water use. We combined expression analysis of the
                      two paralogs in untreated and ABA-treated leaves with the
                      functional characterization of the two genes using grapevine
                      epfl9-1 and epfl9-2 mutants. A physiological analysis of
                      epfl9-2 mutants under different environmental conditions was
                      also performed. We showed that VviEPFL9-1 is exclusively
                      expressed in leaf primordia, whereas VviEPFL9-2 plays a
                      predominant role in fine-tuning SD during the leaf
                      expansion. An epfl9-2 mutant line with $84\%$ lower SD than
                      wild type, exhibited a significant improvement in intrinsic
                      water-use efficiency under both well-watered and
                      water-stressed conditions, with little trade-off in
                      photosynthesis. When the reduction in SD was close to
                      $60\%,$ photosynthetic rate and stomatal conductance were
                      comparable to WT. Our results provide compelling evidence
                      that VviEPFL9-2 knockout determines a significant reduction
                      in stomatal density without a major impact on photosynthesis
                      which may help optimize the adverse impacts of climate
                      change on viticulture.},
      cin          = {IBG-2},
      ddc          = {580},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {40775479},
      UT           = {WOS:001545791200001},
      doi          = {10.1007/s00299-025-03577-9},
      url          = {https://juser.fz-juelich.de/record/1044971},
}