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@ARTICLE{Engelhorn:1044973,
author = {Engelhorn, Julia and Snodgrass, Samantha J. and Kok, Amelie
and Seetharam, Arun S. and Schneider, Michael and Kiwit,
Tatjana and Singh, Ayush and Banf, Michael and Doan, Duong
Thi Hai and Khaipho-Burch, Merritt and Runcie, Daniel E. and
Sánchez-Camargo, Victor A. and Bader, Rechien and Vladimir
Torres-Rodriguez, J. and Sun, Guangchao and Stam, Maike and
Fiorani, Fabio and Beier, Sebastian and Schnable, James C.
and Bass, Hank W. and Hufford, Matthew B. and Stich,
Benjamin and Frommer, Wolf B. and Ross-Ibarra, Jeffrey and
Hartwig, Thomas},
title = {{G}enetic variation at transcription factor binding sites
largely explains phenotypic heritability in maize},
journal = {Nature genetics},
volume = {57},
issn = {1061-4036},
address = {London},
publisher = {Macmillan Publishers Limited, part of Springer Nature},
reportid = {FZJ-2025-03463},
pages = {2313–2322},
year = {2025},
abstract = {Comprehensive maps of functional variation at transcription
factor (TF) binding sites (cis-elements) are crucial for
elucidating how genotype shapes phenotype. Here, we report
the construction of a pan-cistrome of the maize leaf under
well-watered and drought conditions. We quantified
haplotype-specific TF footprints across a pan-genome of 25
maize hybrids and mapped over 200,000 variants, genetic,
epigenetic, or both (termed binding quantitative trait loci
(bQTL)), linked to cis-element occupancy. Three lines of
evidence support the functional significance of bQTL: (1)
coincidence with causative loci that regulate traits,
including vgt1, ZmTRE1 and the MITE transposon near ZmNAC111
under drought; (2) bQTL allelic bias is shared between
inbred parents and matches chromatin immunoprecipitation
sequencing results; and (3) partitioning genetic variation
across genomic regions demonstrates that bQTL capture the
majority of heritable trait variation across $~72\%$ of 143
phenotypes. Our study provides an auspicious approach to
make functional cis-variation accessible at scale for
genetic studies and targeted engineering of complex traits.},
cin = {IBG-2},
ddc = {570},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {2171 - Biological and environmental resources for
sustainable use (POF4-217)},
pid = {G:(DE-HGF)POF4-2171},
typ = {PUB:(DE-HGF)16},
pubmed = {40789919},
UT = {WOS:001548254400001},
doi = {10.1038/s41588-025-02246-7},
url = {https://juser.fz-juelich.de/record/1044973},
}