TY  - JOUR
AU  - Till, Katharina
AU  - Seinen, Anne-Bart
AU  - Wruck, Florian
AU  - Sunderlikova, Vanda
AU  - Galmozzi, Carla V.
AU  - Katranidis, Alexandros
AU  - Bukau, Bernd
AU  - Kramer, Günter
AU  - Tans, Sander J.
TI  - Trigger factor accelerates nascent chain compaction and folding
JO  - Proceedings of the National Academy of Sciences of the United States of America
VL  - 122
IS  - 30
SN  - 0027-8424
CY  - Washington, DC
PB  - National Acad. of Sciences
M1  - FZJ-2025-03475
SP  - e2422678122
PY  - 2025
AB  - Recent work indicates that many chaperones bind protein chains already during their translation by ribosomes. While chaperones are thought to merely “hold” the nascent protein chains, current methods cannot study their conformational changes. We simultaneously image single chaperone binding and detect nascent protein conformation. We show that the chaperone trigger factor accelerates the folding of proteins as they emerge from the ribosome and reveal the mechanism: By enhancing the polypeptide collapse, it pushes residues together. Our mechanism promotes folding to occur cotranslationally, impacts the many processes that depend on it, like cotranslational protein assembly, translation arrest mitigation, and aggregation suppression, and can help explain how trigger factor interacts with downstream chaperones and how cells produce proteins with limited errors.
LB  - PUB:(DE-HGF)16
C6  - 40711920
UR  - <Go to ISI:>//WOS:001544079300001
DO  - DOI:10.1073/pnas.2422678122
UR  - https://juser.fz-juelich.de/record/1044990
ER  -