Home > Publications database > In vivo and in vitro validation of reference tissue models for the mGluR5 ligand (11C)ABP688 > print

001     10453
005     20210129210520.0
024 7 _ |2 pmid
|a pmid:20531460
024 7 _ |2 pmc
|a pmc:PMC2949244
024 7 _ |2 DOI
|a 10.1038/jcbfm.2010.65
024 7 _ |2 WOS
|a WOS:000280561900013
037 _ _ |a PreJuSER-10453
041 _ _ |a eng
082 _ _ |a 610
084 _ _ |2 WoS
|a Endocrinology & Metabolism
084 _ _ |2 WoS
|a Hematology
084 _ _ |2 WoS
|a Neurosciences
100 1 _ |a Elmenhorst, D.
|b 0
|u FZJ
|0 P:(DE-Juel1)131679
245 _ _ |a In vivo and in vitro validation of reference tissue models for the mGluR5 ligand (11C)ABP688
260 _ _ |a [s.l.]
|b Ovid
|c 2010
300 _ _ |a 1538 - 1549
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |a Journal of Cerebral Blood Flow and Metabolism
|x 0271-678X
|0 3137
|v 30
500 _ _ |a This study was supported by the Alzheimer's Association new investigator grant, the Heinrich Hertz Foundation of the Ministry of Science and Technology, North-Rhine Westfalia, Germany (DE), Fonds de la Recherche en Sante du Quebec (FRSQ), Chercheur Burcier Award, Nussia and Andre Aisenstadt Foundation and Fondation Savoy.
520 _ _ |a The primary objective of this study was to verify the suitability of reference tissue-based quantification methods of the metabotropic glutamate receptor type 5 (mGluR(5)) with [(11)C]ABP688. This study presents in vivo (Positron Emission Tomography (PET)) and in vitro (autoradiography) measurements of mGluR(5) densities in the same rats and evaluates both noninvasive and blood-dependent pharmacokinetic models for the quantification of [(11)C]ABP688 binding. Eleven rats underwent [(11)C]ABP688 PET scans. In five animals, baseline scans were compared with blockade experiments with the antagonist 1,2-methyl-6-(phenylethynyl)-pyridine (MPEP), and arterial blood samples were drawn and corrected for metabolites. Afterward, saturation-binding autoradiography was performed. Blocking with MPEP resulted in an average decrease of the total distribution volume (V(T)) between 43% and 58% (thalamus and caudate-putamen, respectively) but had no significant effect on cerebellar V(T) (mean reduction: -0.01%). Comparing binding potential (BP(ND)) based on the V(T) with noninvasively determined BP(ND) revealed an average negative bias of 0.7% in the caudate-putamen and an average positive bias of 3.1% in the low-binding regions. Scan duration of 50 minutes is required. The cerebellum is a suitable reference region for the quantification of mGluR(5) availability as measured with [(11)C]ABP688 PET in rats. Blood-based and reference region-based PET quantification shows a significant linear relationship to autoradiographic determinations.
536 _ _ |0 G:(DE-Juel1)FUEK409
|2 G:(DE-HGF)
|x 0
|c FUEK409
|a Funktion und Dysfunktion des Nervensystems (FUEK409)
536 _ _ |a 89574 - Theory, modelling and simulation (POF2-89574)
|0 G:(DE-HGF)POF2-89574
|c POF2-89574
|x 1
|f POF II T
588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Animals
650 _ 2 |2 MeSH
|a Autoradiography: methods
650 _ 2 |2 MeSH
|a Binding Sites
650 _ 2 |2 MeSH
|a Brain: metabolism
650 _ 2 |2 MeSH
|a Brain: radionuclide imaging
650 _ 2 |2 MeSH
|a Carbon Radioisotopes: analysis
650 _ 2 |2 MeSH
|a Carbon Radioisotopes: metabolism
650 _ 2 |2 MeSH
|a Carbon Radioisotopes: pharmacokinetics
650 _ 2 |2 MeSH
|a Kinetics
650 _ 2 |2 MeSH
|a Male
650 _ 2 |2 MeSH
|a Oximes: analysis
650 _ 2 |2 MeSH
|a Oximes: metabolism
650 _ 2 |2 MeSH
|a Oximes: pharmacokinetics
650 _ 2 |2 MeSH
|a Positron-Emission Tomography: methods
650 _ 2 |2 MeSH
|a Pyridines: analysis
650 _ 2 |2 MeSH
|a Pyridines: metabolism
650 _ 2 |2 MeSH
|a Pyridines: pharmacokinetics
650 _ 2 |2 MeSH
|a Rats
650 _ 2 |2 MeSH
|a Rats, Sprague-Dawley
650 _ 2 |2 MeSH
|a Receptors, Metabotropic Glutamate: analysis
650 _ 2 |2 MeSH
|a Receptors, Metabotropic Glutamate: metabolism
650 _ 7 |0 0
|2 NLM Chemicals
|a 3-(6-methylpyridin-2-ylethynyl)cyclohex-2-enone-O-methyloxime
650 _ 7 |0 0
|2 NLM Chemicals
|a Carbon Radioisotopes
650 _ 7 |0 0
|2 NLM Chemicals
|a Oximes
650 _ 7 |0 0
|2 NLM Chemicals
|a Pyridines
650 _ 7 |0 0
|2 NLM Chemicals
|a Receptors, Metabotropic Glutamate
650 _ 7 |0 0
|2 NLM Chemicals
|a metabotropic glutamate receptor 5
650 _ 7 |a J
|2 WoSType
653 2 0 |2 Author
|a autoradiography
653 2 0 |2 Author
|a blocking
653 2 0 |2 Author
|a kinetic modeling
653 2 0 |2 Author
|a positron emission tomography
653 2 0 |2 Author
|a [C-11]ABP688
700 1 _ |a Minuzzi, L.
|b 1
|0 P:(DE-HGF)0
700 1 _ |a Aliaga, A.
|b 2
|0 P:(DE-HGF)0
700 1 _ |a Rowley, J.
|b 3
|0 P:(DE-HGF)0
700 1 _ |a Massarweh, G.
|b 4
|0 P:(DE-HGF)0
700 1 _ |a Diksic, M.
|b 5
|0 P:(DE-HGF)0
700 1 _ |a Bauer, A.
|b 6
|u FZJ
|0 P:(DE-Juel1)131672
700 1 _ |a Rosa-Neto, P.
|b 7
|0 P:(DE-HGF)0
773 _ _ |a 10.1038/jcbfm.2010.65
|g Vol. 30, p. 1538 - 1549
|p 1538 - 1549
|q 30<1538 - 1549
|0 PERI:(DE-600)2039456-1
|t Journal of cerebral blood flow & metabolism
|v 30
|y 2010
|x 0271-678X
856 7 _ |2 Pubmed Central
|u http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949244
909 C O |o oai:juser.fz-juelich.de:10453
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914 1 _ |y 2010
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