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@MISC{Scherr:1045534,
author = {Scherr, Tim and Seiffarth, Johannes and Wollenhaupt,
Bastian and Neumann, Oliver and Schilling, Marcel P. and
Kohlheyer, Dietrich and Scharr, Hanno and Nöh, Katharina
and Mikut, Ralf},
title = {microbe{SEG} dataset},
publisher = {Zenodo},
reportid = {FZJ-2025-03537},
year = {2022},
abstract = {B. subtilis and E. coli cell segmentation dataset
consisting of test data annotated by three experts ( test ),
data annotated manually by a single microbeSEG user within
30 minutes ( 30min-man ), data annotated manually by a
single microbeSEG user within 30 minutes and data annotated
with microbeSEG pre-labeling with 15 minutes manual
correction time ( $30min-man_15min-pre$ , includes the
30min-man dataset). Images, instance segmentation masks and
image-segmentation overlays are provided. All images are
crops of size 320px x 320px. Annotations were made with
ObiWan-Microbi . Data acquisition The phase contrast images
of growing B. subtilis and E. coli colonies were acquired
with a fully automated time-lapse microscope setup (TI
Eclipse, Nikon, Germany) using a 100x oil immersion
objective (Plan Apochromat λ Oil, N.A. 1.45, WD 170 µm,
Nikon Microscopy). Time-lapse images were taken every 15
minutes for B. subtilis and every 20 minutes for E. coli .
Cultivation took place inside a special microfluidic
cultivation device. Resolution: 0.07μm/px for B. subtilis
und 0.09μm/px for E. coli . microbeSEG import For the use
with microbeSEG , create or select a new training set within
the software and use the training data import functionality.
Best import train data with the 'train' checkbox checked,
validation data with the 'val' checkbox checked, and test
data with the 'test' checkbox checked. Since the images are
already normalized, the 'keep normalization' functionality
can be used.},
keywords = {cell segmentation (Other) / omero (Other) / microbeSEG
(Other)},
cin = {IBG-1 / IAS-8},
cid = {I:(DE-Juel1)IBG-1-20101118 / I:(DE-Juel1)IAS-8-20210421},
pnm = {2171 - Biological and environmental resources for
sustainable use (POF4-217)},
pid = {G:(DE-HGF)POF4-2171},
typ = {PUB:(DE-HGF)32},
doi = {10.5281/ZENODO.6497714},
url = {https://juser.fz-juelich.de/record/1045534},
}