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@ARTICLE{Woods:1045721,
      author       = {Woods, Eric V and Schwarz, Tim M and Singh, Mahander P and
                      Zhang, Shuo and Kim, Se-Ho and El-Zoka, Ayman A and Gremer,
                      Lothar and Willbold, Dieter and McCarroll, Ingrid and Gault,
                      Baptiste},
      title        = {{M}apping the {P}ath to {C}ryogenic {A}tom {P}robe
                      {T}omography {A}nalysis of {B}iomolecules},
      journal      = {Microscopy and microanalysis},
      volume       = {31},
      number       = {4},
      issn         = {1079-8501},
      address      = {Oxford},
      publisher    = {Oxford University Press},
      reportid     = {FZJ-2025-03565},
      pages        = {ozaf032},
      year         = {2025},
      abstract     = {The understanding of protein structure and interactions
                      remains a fundamental challenge in modern biology. While
                      X-ray and electron-based techniques have provided
                      atomic-level protein configurations, they require numerous
                      molecules for averaged views and lack detailed compositional
                      information crucial for biochemical activity. Atom probe
                      tomography (APT) emerges as a promising tool for biological
                      material analysis, though its capabilities for examining
                      biomolecules in their native, hydrated state remain largely
                      unexplored. We present systematic analyses of amino acids in
                      frozen aqueous solutions using two different nanoporous
                      metal supports across various analysis conditions. Our
                      methodology employs a complete cryogenic workflow, including
                      drop-casting, inert gas glovebox freezing, and specimen
                      transfer via a cryogenically cooled ultra-high vacuum
                      shuttle to both focused ion beam microscopy and atom probes.
                      Using water molecular ion ratios as electrostatic field
                      condition indicators, we investigate amino acid
                      fragmentation and behavior. We evaluate the critical factors
                      for successful biomolecular analysis: support material
                      selection, cryogenic specimen preparation, and optimal data
                      acquisition parameters. This work establishes guidelines for
                      cryogenic APT analysis of biomolecules, advancing the
                      technique's application in biological sciences.},
      cin          = {IBI-7},
      ddc          = {500},
      cid          = {I:(DE-Juel1)IBI-7-20200312},
      pnm          = {5244 - Information Processing in Neuronal Networks
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5244},
      typ          = {PUB:(DE-HGF)16},
      doi          = {10.1093/mam/ozaf032},
      url          = {https://juser.fz-juelich.de/record/1045721},
}