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@ARTICLE{Vitko:1045962,
      author       = {Vitko, Sandra and Tokić, Mirta and Braun, Silvia and
                      Brehm, Thorsten and Pavlović, Iva and Fiorani, Fabio and
                      Novák, Ondřej and Bauer, Nataša and Leljak-Levanić,
                      Dunja and Vidaković-Cifrek, Željka},
      title        = {{H}eat tolerance in {A}rabidopsis thaliana seedlings
                      requires functional {DMS}3, a component of de novo
                      methylation},
      journal      = {Plant stress},
      volume       = {18},
      issn         = {2667-064X},
      address      = {Amsterdam},
      publisher    = {Elsevier},
      reportid     = {FZJ-2025-03629},
      pages        = {101013 -},
      year         = {2025},
      abstract     = {The protein Defective in RNA-directed DNA Methylation 3
                      (DMS3) is part of RNA-directed DNA methylation, an
                      epigenetic mechanism involved in the regulation of plant
                      development and stress response. However, the specific role
                      of the DMS3 protein in thermotolerance remains unclear. To
                      determine how altered DMS3 expression and functionality
                      affects thermotolerance, DMS3-overexpressor (oeDMS3),
                      DMS3-mutant (dms3-1) and wild-type Arabidopsis thaliana
                      seedlings were heat-treated and analyzed, focusing on
                      morphological, physiological, biochemical and molecular
                      changes. The dms3-1 line showed the highest
                      thermosensitivity after short-term exposure to 45 °C for
                      45 min. However, both dms3-1 and oeDMS3 showed a greater
                      reduction in morphological traits compared to wild type
                      after exposure to 40 °C for 6 h. Hormonal profiling
                      showed that the dms3-1 and oeDMS3 lines had similar hormonal
                      profiles characterized by lower jasmonate levels compared to
                      wild type, both under stress and control conditions. The
                      heat-stressed dms3-1 line contained increased cytokinin
                      levels predominantly in the form of ribosides, and also
                      accumulated inactive auxin metabolites. Exposure to 37 °C
                      for 24 h destabilized and altered the localization of the
                      DMS3 protein in the root tissue. After exposure to 37 °C
                      for 6 h, the dms3-1 line showed a delayed recovery of
                      reduced photosynthetic efficiency, accompanied by a partial
                      activation of the antioxidant system and increased proline
                      content. Under control conditions, dms3-1 plants exhibited
                      reduced growth and lower expression of RuBisCO, HSP90 and
                      HSP70 proteins. Overall, our results suggest a crucial role
                      of DMS3 in thermotolerance, hormone balance, antioxidant
                      defense and photosynthetic efficiency, indicating the
                      importance of a functional and balanced DMS3 protein for
                      thermotolerance and for plant growth and development under
                      control conditions.},
      cin          = {IBG-2},
      ddc          = {580},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217) / EPPN2020 - European Plant
                      Phenotyping Network 2020 (731013)},
      pid          = {G:(DE-HGF)POF4-2171 / G:(EU-Grant)731013},
      typ          = {PUB:(DE-HGF)16},
      doi          = {10.1016/j.stress.2025.101013},
      url          = {https://juser.fz-juelich.de/record/1045962},
}