TY  - JOUR
AU  - Beier, Sebastian
AU  - Bolger, Marie Elizabeth
AU  - Chanvivattana, Yindee
AU  - Bolger, Anthony Michael
AU  - Schmidt, Maximilian Heinrich-Wilhelm
AU  - Leelapon, Oranuch
AU  - Rakmit, Rungroj
AU  - Changmai, Kulnida
AU  - Ruayreun, Ratiporn
AU  - Srithundon, Sukanya
AU  - Totaiya, Duangjit
AU  - Sitchanukrit, Boriphat
AU  - Usadel, Björn
AU  - Wongtiem, Prapit
AU  - Amawan, Suwaluk
AU  - Wojciechowski, Tobias
TI  - Chromosome-level genome assemblies of Thai cassava ecotypes (Manihot esculenta & Manihot glaziovii)
JO  - Scientific data
VL  - 12
IS  - 1
SN  - 2052-4436
CY  - London
PB  - Nature Publ. Group
M1  - FZJ-2025-03935
SP  - 1591
PY  - 2025
AB  - Cassava is a vital staple crop, yet genomic resources for diverse ecotypes, particularly from key regions, remain limited. To address this, we generated high-quality genome assemblies for nine Thai M. esculenta cultivars and one wild relative, Manihot glaziovii. The sequencing strategy combined Oxford Nanopore long reads for initial assembly with Illumina short reads for polishing and quality assessment. For five of the genotypes, extensive RNA-Seq data from various tissues and developmental stages were also produced to guide gene annotation. We provide detailed technical validation of the ten genome assemblies, reporting on key metrics of contiguity (N50s from 28.9 to 35.2 Mb), completeness (Complete BUSCO scores from 95.69% to 99.21%), and base-level accuracy (k-mer QV scores from 33.47 to 37.67). The final annotated assemblies and all raw sequencing data have been deposited in public archives and are readily accessible. These datasets represent a significant expansion of the genomic toolkit for Asian cassava, providing a foundational resource for future genetic discovery, comparative genomics, and advanced breeding applications.
LB  - PUB:(DE-HGF)16
DO  - DOI:10.1038/s41597-025-05998-3
UR  - https://juser.fz-juelich.de/record/1046723
ER  -