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@ARTICLE{Shaikh:1047310,
      author       = {Shaikh, Imran G. and Kostritskii, Andrei Y. and Renigunta,
                      Aparna and Jeschke, Julia and Halaszovich, Christian R. and
                      Zhao, Wencai and Geissler, Marc and Bhushan, Sudhanshu and
                      Weber, Stefanie and Meinhardt, Andreas and Machtens,
                      Jan-Philipp and Oliver, Dominik and Renigunta, Vijay},
      title        = {{E}lectrochemical coupling at the plasma membrane by mouse
                      voltage-sensitive phosphatase requires association with
                      basigin},
      journal      = {Cell reports},
      volume       = {44},
      number       = {9},
      issn         = {2211-1247},
      address      = {Maryland Heights, MO},
      publisher    = {Cell Press},
      reportid     = {FZJ-2025-04225},
      pages        = {116200 -},
      year         = {2025},
      note         = {We thank Olga Ebers, Galina Zielke, and Nesli Oezen for
                      excellent technical assistance.We gratefully acknowledge the
                      gifts of mVSP from S.M. Bajjalieh, Ci-VSPand Dr-VSP from Y.
                      Okamura, Xl-VSP from W. Ratzan, NPTN ERret from B.
                      Fakler,PLCδ1-PH from T. Balla, Lyn11 from T. Meyer,
                      tubby-Cterm from L. Shapiro, andTAPP1-PH from D. Alessi.
                      This work was supported by grants from the von Behring-Ro¨
                      ntgen-Stiftung (65-0028) to D.O., V.R., A.M., and S.B.; from
                      the DeutscheForschungsgemeinschaft (DFG; German Research
                      Foundation) to V.R. (RE 4617/2-1), D.O. (OL 240/8-1 as part
                      of the Research Unit FOR 5046, project P3), andJ.-P.M. (MA
                      7525/2-2, as part of the Research Unit FOR 5046, project
                      P2); andby the LOEWE Research Focus CoroPan (Project P5),
                      funded by the State ofHesse, Germany (to V.R. and A.R.) The
                      authors gratefully acknowledge thecomputing time granted
                      through JARA on the supercomputer JURECA at
                      ForschungszentrumJu¨ lich under grant mpogt.},
      abstract     = {Voltage-sensitive phosphatases (VSPs) are unique enzymes
                      that mediate electrochemical coupling by
                      convertingphosphoinositides in response to membrane
                      depolarization. In mammals, VSPs are involved in
                      regulatingsperm motility. The basic functionality of
                      mammalian VSPs has remained enigmatic, as retention
                      tointracellular compartments precluded functional analysis.
                      Here, a membrane yeast two-hybrid assay identifiesbasigin as
                      an accessory subunit of mouse VSP (mVSP). Co-expression with
                      basigin or its homologsinduced trafficking of mVSP from the
                      endoplasmic reticulum to the plasma membrane. Mutational
                      analysisand structural predictions by AlphaFold-Multimer
                      showed that the functional effect on mVSP requires
                      interactionof the transmembrane region of basigin with the
                      voltage sensor domain of mVSP. Basigin-mediatedsurface
                      localization allowed for functional analysis, revealing that
                      mVSP acts as a voltage-activated5-phosphatase against
                      PI(4,5)P 2 and PI(3,4,5)P 3 with a more negative activation
                      range compared to nonmammalianVSPs.},
      cin          = {IBI-1},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBI-1-20200312},
      pnm          = {5241 - Molecular Information Processing in Cellular Systems
                      (POF4-524) / DFG project G:(GEPRIS)426950122 - FOR 5046:
                      Integrative Analyse epithelialer SLC26 Anionentransporter
                      – von der molekularen Struktur zur Pathophysiologie
                      (426950122)},
      pid          = {G:(DE-HGF)POF4-5241 / G:(GEPRIS)426950122},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {40880230},
      UT           = {WOS:001564218300001},
      doi          = {10.1016/j.celrep.2025.116200},
      url          = {https://juser.fz-juelich.de/record/1047310},
}