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@ARTICLE{Schmidt:1047475,
author = {Schmidt, Patrick and Lindemeyer, Johannes and Raut, Pranali
and Schütz, Markus and Saniternik, Sven and Jönsson,
Jannika and Endepols, Heike and Fischer, Thomas and Quaas,
Alexander and Schlößer, Hans Anton and Thelen, Martin and
Grüll, Holger},
title = {{M}ultiparametric {C}haracterization of the {DSL}-6{A}/{C}1
{P}ancreatic {C}ancer {M}odel in {R}ats},
journal = {Cancers},
volume = {16},
number = {8},
issn = {2072-6694},
address = {Basel},
publisher = {MDPI},
reportid = {FZJ-2025-04326},
pages = {1535},
year = {2024},
note = {This research was supported by the German Federal Ministry
of Education and Research (BMBF), grant number 13GW0364D
(“MR-HIFU Pancreas”).},
abstract = {The DSL-6A/C1 murine pancreatic ductal adenocarcinoma
(PDAC) tumor model was established in Lewis rats and
characterized through a comprehensive multiparametric
analysis to compare it to other preclinical tumor models and
explore potential diagnostic and therapeutical targets.
DSL-6A/C1 tumors were histologically analyzed to elucidate
PDAC features. The tumor microenvironment was studied for
immune cell prevalence. Multiparametric MRI and PET imaging
were utilized to characterize tumors, and
68Ga-FAPI-46-targeting cancer-associated fibroblasts (CAFs),
were used to validate the histological findings. The
histology confirmed typical PDAC characteristics, such as
malformed pancreatic ductal malignant cells and CAFs.
Distinct immune landscapes were identified, revealing an
increased presence of CD8+ T cells and a decreased CD4+ T
cell fraction within the tumor microenvironment. PET imaging
with 68Ga-FAPI tracers exhibited strong tracer uptake in
tumor tissues. The MRI parameters indicated increasing
intralesional necrosis over time and elevated contrast media
uptake in vital tumor areas. We have demonstrated that the
DSL-6A/C1 tumor model, particularly due to its high
tumorigenicity, tumor size, and 68Ga-FAPI-46 sensitivity, is
a suitable alternative to established small animal models
for many forms of preclinical analyses and therapeutic
studies of PDAC.},
cin = {INM-5},
ddc = {610},
cid = {I:(DE-Juel1)INM-5-20090406},
pnm = {5253 - Neuroimaging (POF4-525)},
pid = {G:(DE-HGF)POF4-5253},
typ = {PUB:(DE-HGF)16},
doi = {10.3390/cancers16081535},
url = {https://juser.fz-juelich.de/record/1047475},
}