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@ARTICLE{Berkamp:1048830,
      author       = {Berkamp, Sabrina and Jungbluth, Lisa and Katranidis,
                      Alexandros and Mostafavi, Siavash and Korculanin, Olivera
                      and Lu, Peng-Han and Ickert, Lotte and Dierig, Maya M. and
                      Sharma, Lokesh and Thukral, Lipi and Huesgen, Pitter F. and
                      Kononenko, Natalia L. and Fitter, Jörg and Dunin-Borkowski,
                      Rafal E. and Sachse, Carsten},
      title        = {{S}tructural organization of p62 filaments and the cellular
                      ultrastructure of calcium-rich p62-enwrapped lipid droplet
                      cargo},
      journal      = {Nature Communications},
      volume       = {16},
      number       = {1},
      issn         = {2041-1723},
      address      = {[London]},
      publisher    = {Springer Nature},
      reportid     = {FZJ-2025-04938},
      pages        = {10810},
      year         = {2025},
      abstract     = {The selective autophagy receptor p62/SQSTM1 is known to
                      form higher-order filaments in vitro and to undergo
                      liquid-liquid phase separation when mixed with
                      poly-ubiquitin. Here, we determine the full-length cryo-EM
                      structure of p62 and elucidate a structured double helical
                      filament scaffold composed of the PB1-domain associated with
                      the flexible C-terminal part and the solvent-accessible
                      major groove. At different pH values and upon binding to
                      soluble LC3, LC3-conjugated membranes and poly-ubiquitin, we
                      observe p62 filament re-arrangements in the form of
                      structural unwinding, disassembly, lateral association and
                      bundling, respectively. In the cellular environment, under
                      conditions of ATG5 knockdown leading to stalled autophagy,
                      we imaged high-contrast layers consisting of p62 oligomers
                      enwrapping lipid droplets by cryogenic electron tomography
                      in situ, which we identified as calcium as well as
                      phosphorus by compositional spectroscopy analysis. Together,
                      we visualize the cellular ultrastructure of p62 oligomers
                      with high calcium content as a potential early stage of
                      autophagy.},
      cin          = {ER-C-3},
      ddc          = {500},
      cid          = {I:(DE-Juel1)ER-C-3-20170113},
      pnm          = {5241 - Molecular Information Processing in Cellular Systems
                      (POF4-524) / 5352 - Understanding the Functionality of Soft
                      Matter and Biomolecular Systems (POF4-535)},
      pid          = {G:(DE-HGF)POF4-5241 / G:(DE-HGF)POF4-5352},
      typ          = {PUB:(DE-HGF)16},
      doi          = {10.1038/s41467-025-66785-7},
      url          = {https://juser.fz-juelich.de/record/1048830},
}