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001050624 005__ 20260115203947.0
001050624 0247_ $$2datacite_doi$$a10.34734/FZJ-2026-00376
001050624 037__ $$aFZJ-2026-00376
001050624 041__ $$aEnglish
001050624 1001_ $$0P:(DE-Juel1)165155$$aArsova, Borjana$$b0$$eCorresponding author$$ufzj
001050624 1112_ $$a18th Symposium on biological nitrogen fixation with non-legumes$$cOxford, UK$$d2025-08-30 - 2025-08-30$$wUK
001050624 245__ $$aUnderstanding the physiological changes behind bacteria-enhanced growth of Brachypodium under nitrogen stress
001050624 260__ $$c2025
001050624 3367_ $$033$$2EndNote$$aConference Paper
001050624 3367_ $$2DataCite$$aOther
001050624 3367_ $$2BibTeX$$aINPROCEEDINGS
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001050624 3367_ $$0PUB:(DE-HGF)6$$2PUB:(DE-HGF)$$aConference Presentation$$bconf$$mconf$$s1768462223_3993$$xPlenary/Keynote
001050624 500__ $$aThe 18th Symposium on biological nitrogen fixation with non-legumes is held as a satellite meeting to the ENFC.
001050624 520__ $$aNon-nodulating plants are some of the most widely grown crops today.  Exploiting their interactions with bacteria as plant growth promoters, could lead to more sustainable agriculture. Bacteria with genomic predisposition to nitrogen (N) fixation are of interest for reducing the environmental impact associated with N-fertilizers.Our work uses the cereal model Brachypodium dystachion and the bacteria Herbaspirillum seropedicae (Hs) [1] and Pseudomonas koreensis (Pk) [2]. Brachypodium’s time-resolved physiological and molecular changes to the presence of a bacterium showed the plasticity of the plant system under bacterial modulation, at limiting N. The work was performed in easy to reproduce gnotobiotic systems, and integrated noninvasive plant phenotyping, elemental analysis, δ15N measurements, proteomics and lipidomics. In both cases we found recovery of N-deficiency symptoms about two weeks post-inoculation, with increased N content in inoculated plants. The association with Hs pointed to two modes of plant-microbe interaction (PMI) dependant on N availability, with indication of N-fixation at low N. Association with Pk resulted in decreased C in inoculated roots, indicating nutrient exchanges between Brachypodium and Pk. A trend toward decreased δ15N signatures did not fully confirm N-fixation by Pk, although Pk grew on N-free medium in vitro. Importantly, proteomic and lipidomic changes were detected in Brachypodium inoculated with Pk. Proteomic shifts were driven by both N availability and Pk, bringing central N metabolism proteins of low N inoculated plants to levels similar of high N plants. Lipidomic changes responded to N limitation alone.Thus, associative PMI offer multiple strategies to mitigate plant abiotic stress, and the potential molecular mechanisms will be discussed in this presentation.References 1.	Kuang et al., (2022). Journal of Experimental Botany, vol 73, 5306–5321.2.	Sanow et al., (2023). Molecular Plant-Microbe Interactions, vol 36, 536–548.
001050624 536__ $$0G:(DE-HGF)POF4-2171$$a2171 - Biological and environmental resources for sustainable use (POF4-217)$$cPOF4-217$$fPOF IV$$x0
001050624 8564_ $$uhttps://web.cvent.com/event/aa2ad978-6a8e-4c90-8d6b-63fd30ac8447/summary
001050624 8564_ $$uhttps://juser.fz-juelich.de/record/1050624/files/abstract_Arsova_ENFC_2025.doc$$yOpenAccess
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