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@INPROCEEDINGS{Arsova:1050624,
      author       = {Arsova, Borjana},
      title        = {{U}nderstanding the physiological changes behind
                      bacteria-enhanced growth of {B}rachypodium under nitrogen
                      stress},
      reportid     = {FZJ-2026-00376},
      year         = {2025},
      note         = {The 18th Symposium on biological nitrogen fixation with
                      non-legumes is held as a satellite meeting to the ENFC.},
      abstract     = {Non-nodulating plants are some of the most widely grown
                      crops today. Exploiting their interactions with bacteria as
                      plant growth promoters, could lead to more sustainable
                      agriculture. Bacteria with genomic predisposition to
                      nitrogen (N) fixation are of interest for reducing the
                      environmental impact associated with N-fertilizers.Our work
                      uses the cereal model Brachypodium dystachion and the
                      bacteria Herbaspirillum seropedicae (Hs) [1] and Pseudomonas
                      koreensis (Pk) [2]. Brachypodium’s time-resolved
                      physiological and molecular changes to the presence of a
                      bacterium showed the plasticity of the plant system under
                      bacterial modulation, at limiting N. The work was performed
                      in easy to reproduce gnotobiotic systems, and integrated
                      noninvasive plant phenotyping, elemental analysis, δ15N
                      measurements, proteomics and lipidomics. In both cases we
                      found recovery of N-deficiency symptoms about two weeks
                      post-inoculation, with increased N content in inoculated
                      plants. The association with Hs pointed to two modes of
                      plant-microbe interaction (PMI) dependant on N availability,
                      with indication of N-fixation at low N. Association with Pk
                      resulted in decreased C in inoculated roots, indicating
                      nutrient exchanges between Brachypodium and Pk. A trend
                      toward decreased δ15N signatures did not fully confirm
                      N-fixation by Pk, although Pk grew on N-free medium in
                      vitro. Importantly, proteomic and lipidomic changes were
                      detected in Brachypodium inoculated with Pk. Proteomic
                      shifts were driven by both N availability and Pk, bringing
                      central N metabolism proteins of low N inoculated plants to
                      levels similar of high N plants. Lipidomic changes responded
                      to N limitation alone.Thus, associative PMI offer multiple
                      strategies to mitigate plant abiotic stress, and the
                      potential molecular mechanisms will be discussed in this
                      presentation.References 1. Kuang et al., (2022). Journal of
                      Experimental Botany, vol 73, 5306–5321.2. Sanow et al.,
                      (2023). Molecular Plant-Microbe Interactions, vol 36,
                      536–548.},
      month         = {Aug},
      date          = {2025-08-30},
      organization  = {18th Symposium on biological nitrogen
                       fixation with non-legumes, Oxford, UK
                       (UK), 30 Aug 2025 - 30 Aug 2025},
      subtyp        = {Plenary/Keynote},
      cin          = {IBG-2},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)6},
      doi          = {10.34734/FZJ-2026-00376},
      url          = {https://juser.fz-juelich.de/record/1050624},
}