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@INPROCEEDINGS{Sanow:1050625,
      author       = {Sanow, Stefan and Kelm, Jana and Huesgen, Pitter and
                      Schaaf, Gabriel and Watt, Michelle and Arsova, Borjana},
      title        = {{M}olecular {S}trategies {U}nderlying
                      {P}seudomonas-{E}nhanced {G}rowth in {N}itrogen-{S}tressed
                      {B}rachypodium},
      reportid     = {FZJ-2026-00377},
      year         = {2025},
      abstract     = {Bacterial association with non-nodulating plants are
                      gaining increasing interest as means to promote more
                      sustainable agricultural practices. In particular, bacteria
                      with genomic predisposition to N-fixation are of interest
                      for reducing the environmental impact associated with
                      N-fertilizers.Pseudomonas koreensis (Pk) has been shown to
                      possess plant growth-promoting properties, but its effects
                      under limited N have not yet been investigated in planta.
                      Furthermore, Brachypodium’s time-resolved molecular
                      acclimatization to the presence of Pk is expected to show
                      the plasticity of the plant system under Pk’s modulation,
                      providing insight for future applications. This study
                      integrated noninvasive root and shoot phenotyping, elemental
                      analysis, δ15N natural abundance, proteomics and
                      lipidomics. Results showed phenotype recovery of
                      N-deficiency symptoms about two weeks post-inoculation, with
                      increased N content in inoculated plants but decreased C in
                      inoculated roots, indicating nutrient exchanges during
                      plant-microbe interaction (PMI). A trend toward decreased
                      δ15N signatures did not fully confirm N-fixation by Pk,
                      although Pk grew on N-free medium in vitro. Importantly,
                      proteomic and lipidomic changes were detected in
                      Brachypodium: proteomic shifts were driven by N limitation
                      and Pk inoculation- bringing central N metabolism protein
                      abundance of low N inoculated plants to levels similar of
                      high N plants. Lipidomic changes responded to nitrogen
                      limitation alone.In conclusion, associative plant-microbe
                      interactions offer multiple strategies to mitigate plant
                      abiotic stress, and the potential molecular mechanisms will
                      be discussed in this presentation.},
      month         = {Jul},
      date          = {2025-07-06},
      organization  = {International Brachypodium Research
                       Conference 2025, Ascona (Switzerland),
                       6 Jul 2025 - 10 Jul 2025},
      subtyp        = {Invited},
      cin          = {IBG-2},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/1050625},
}