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| Home > Publications database > Molecular Strategies Underlying Pseudomonas-Enhanced Growth in Nitrogen-Stressed Brachypodium |
| Home > Publications database > Molecular Strategies Underlying Pseudomonas-Enhanced Growth in Nitrogen-Stressed Brachypodium |
| Conference Presentation (Invited) | FZJ-2026-00377 |
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2025
Abstract: Bacterial association with non-nodulating plants are gaining increasing interest as means to promote more sustainable agricultural practices. In particular, bacteria with genomic predisposition to N-fixation are of interest for reducing the environmental impact associated with N-fertilizers.Pseudomonas koreensis (Pk) has been shown to possess plant growth-promoting properties, but its effects under limited N have not yet been investigated in planta. Furthermore, Brachypodium’s time-resolved molecular acclimatization to the presence of Pk is expected to show the plasticity of the plant system under Pk’s modulation, providing insight for future applications. This study integrated noninvasive root and shoot phenotyping, elemental analysis, δ15N natural abundance, proteomics and lipidomics. Results showed phenotype recovery of N-deficiency symptoms about two weeks post-inoculation, with increased N content in inoculated plants but decreased C in inoculated roots, indicating nutrient exchanges during plant-microbe interaction (PMI). A trend toward decreased δ15N signatures did not fully confirm N-fixation by Pk, although Pk grew on N-free medium in vitro. Importantly, proteomic and lipidomic changes were detected in Brachypodium: proteomic shifts were driven by N limitation and Pk inoculation- bringing central N metabolism protein abundance of low N inoculated plants to levels similar of high N plants. Lipidomic changes responded to nitrogen limitation alone.In conclusion, associative plant-microbe interactions offer multiple strategies to mitigate plant abiotic stress, and the potential molecular mechanisms will be discussed in this presentation.
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