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@ARTICLE{Sanow:1050629,
      author       = {Sanow, S. and Mantz, M. and Wissel, H. and Bhatnagar, A.
                      and Sturm, E. and Kelm, J. M. and Walker, R. and Lücke, A.
                      and Schaaf, G. and Huesgen, P. and Roessner, U. and Watt, M.
                      and Arsova, B.},
      title        = {{S}ystems-level {P}lant {R}esponses {R}eveal {P}seudomonas
                      -{M}ediated {G}rowth {P}romotion in {B}rachypodium {U}nder
                      {N}itrogen {L}imitation},
      reportid     = {FZJ-2026-00381},
      year         = {2025},
      abstract     = {Plant molecular adaptation to plant growth promoting
                      bacteria (PGPB) under nutrient stress remains unclear, yet
                      is essential for advancing PGPB use in agriculture. The
                      model grass Brachypodium dystachion was studied together
                      with Pseudomonas koreeensis (Pk) at two nitrogen (N)
                      conditions. Non-invasive shoot phenotyping showed an
                      immediate response to low-N, while beneficial effects of Pk
                      became quantifiable after day 19. Increased N content in
                      inoculated plants, along with Pk’s ability to grow on
                      N-free media, suggests bacterial N contribution at deficient
                      N. In low-N conditions, Pk-inoculated plants showed $33.2\%$
                      more N than uninoculated controls and biomass comparable to
                      high-N plants. Pk had no effect under sufficient N.
                      Proteomics and lipidomics revealed that lipid profiles were
                      primarily shaped by N availability, while protein abundance
                      responded to both Pk and N status. Inoculated low-N plants
                      displayed protein profiles resembling those of high-N
                      controls, with some distinct exceptions. The plant-microbe
                      interaction is dynamic and developed over 3 weeks, leading
                      to increased biomass and N content. Root proteins strongly
                      induced by Pk under low-N included lipid degradation
                      enzymes, N transporters, and regulatory proteins, suggesting
                      a coordinated remodelling of energy metabolism supporting
                      whole-plant biomass and increased abundance of N uptake
                      proteins.},
      cin          = {IBG-2 / IBG-3},
      cid          = {I:(DE-Juel1)IBG-2-20101118 / I:(DE-Juel1)IBG-3-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)25},
      doi          = {10.1101/2025.11.03.686263},
      url          = {https://juser.fz-juelich.de/record/1050629},
}