001050632 001__ 1050632
001050632 005__ 20260115203947.0
001050632 0247_ $$2doi$$a10.1371/journal.pbio.3003358
001050632 0247_ $$2ISSN$$a1544-9173
001050632 0247_ $$2ISSN$$a1545-7885
001050632 0247_ $$2datacite_doi$$a10.34734/FZJ-2026-00384
001050632 037__ $$aFZJ-2026-00384
001050632 082__ $$a610
001050632 1001_ $$00000-0001-7890-4593$$aNovak, Vlastimil$$b0
001050632 245__ $$aBreaking the reproducibility barrier with standardized protocols for plant–microbiome research
001050632 260__ $$aLawrence, KS$$bPLoS$$c2025
001050632 3367_ $$2DRIVER$$aarticle
001050632 3367_ $$2DataCite$$aOutput Types/Journal article
001050632 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1768465243_3992
001050632 3367_ $$2BibTeX$$aARTICLE
001050632 3367_ $$2ORCID$$aJOURNAL_ARTICLE
001050632 3367_ $$00$$2EndNote$$aJournal Article
001050632 520__ $$aInter-laboratory replicability is crucial yet challenging in microbiome research. Leveraging microbiomes to promote soil health and plant growth requires understanding underlying molecular mechanisms using reproducible experimental systems. In a global collaborative effort involving five laboratories, we aimed to help advance reproducibility in microbiome studies by testing our ability to replicate synthetic community assembly experiments. Our study compared fabricated ecosystems constructed using two different synthetic bacterial communities, the model grass Brachypodium distachyon, and sterile EcoFAB 2.0 devices. All participating laboratories observed consistent inoculum-dependent changes in plant phenotype, root exudate composition, and final bacterial community structure, where Paraburkholderia sp. OAS925 could dramatically shift microbiome composition. Comparative genomics and exudate utilization linked the pH-dependent colonization ability of Paraburkholderia, which was further confirmed with motility assays. The study provides detailed protocols, benchmarking datasets, and best practices to help advance replicable science and inform future multi-laboratory reproducibility studies.
001050632 536__ $$0G:(DE-HGF)POF4-2171$$a2171 - Biological and environmental resources for sustainable use (POF4-217)$$cPOF4-217$$fPOF IV$$x0
001050632 588__ $$aDataset connected to CrossRef, Journals: juser.fz-juelich.de
001050632 7001_ $$0P:(DE-HGF)0$$aAndeer, Peter F.$$b1
001050632 7001_ $$0P:(DE-HGF)0$$aKing, Eoghan$$b2
001050632 7001_ $$0P:(DE-HGF)0$$aCalabria, Jacob$$b3
001050632 7001_ $$aFitzpatrick, Connor$$b4
001050632 7001_ $$0P:(DE-Juel1)164816$$aKelm, Jana M.$$b5
001050632 7001_ $$aWippel, Kathrin$$b6
001050632 7001_ $$aKosina, Suzanne M.$$b7
001050632 7001_ $$aBowen, Benjamin P.$$b8
001050632 7001_ $$aDaum, Chris$$b9
001050632 7001_ $$aZane, Matthew$$b10
001050632 7001_ $$aYadav, Archana$$b11
001050632 7001_ $$aChen, Mingfei$$b12
001050632 7001_ $$aRuss, Dor$$b13
001050632 7001_ $$aAdams, Catharine A.$$b14
001050632 7001_ $$aOwens, Trenton K.$$b15
001050632 7001_ $$aLee, Bradie$$b16
001050632 7001_ $$aDing, Yezhang$$b17
001050632 7001_ $$aSordo, Zineb$$b18
001050632 7001_ $$aChakraborty, Romy$$b19
001050632 7001_ $$aRoux, Simon$$b20
001050632 7001_ $$aDeutschbauer, Adam M.$$b21
001050632 7001_ $$aUshizima, Daniela$$b22
001050632 7001_ $$aZengler, Karsten$$b23
001050632 7001_ $$0P:(DE-Juel1)165155$$aArsova, Borjana$$b24
001050632 7001_ $$aDangl, Jeffery L.$$b25
001050632 7001_ $$aSchulze-Lefert, Paul$$b26
001050632 7001_ $$0P:(DE-Juel1)166460$$aWatt, Michelle$$b27
001050632 7001_ $$0P:(DE-HGF)0$$aVogel, John P.$$b28$$eCorresponding author
001050632 7001_ $$0P:(DE-HGF)0$$aNorthen, Trent R.$$b29$$eCorresponding author
001050632 773__ $$0PERI:(DE-600)2126773-X$$a10.1371/journal.pbio.3003358$$gVol. 23, no. 9, p. e3003358 -$$n9$$pe3003358 -$$tPLoS biology$$v23$$x1544-9173$$y2025
001050632 8564_ $$uhttps://juser.fz-juelich.de/record/1050632/files/journal.pbio.3003358.pdf$$yOpenAccess
001050632 909CO $$ooai:juser.fz-juelich.de:1050632$$popenaire$$popen_access$$pVDB$$pdriver$$pdnbdelivery
001050632 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)164816$$aForschungszentrum Jülich$$b5$$kFZJ
001050632 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)165155$$aForschungszentrum Jülich$$b24$$kFZJ
001050632 9131_ $$0G:(DE-HGF)POF4-217$$1G:(DE-HGF)POF4-210$$2G:(DE-HGF)POF4-200$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF4-2171$$aDE-HGF$$bForschungsbereich Erde und Umwelt$$lErde im Wandel – Unsere Zukunft nachhaltig gestalten$$vFür eine nachhaltige Bio-Ökonomie – von Ressourcen zu Produkten$$x0
001050632 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2024-12-28
001050632 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2024-12-28
001050632 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2024-12-28
001050632 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2024-12-28
001050632 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2024-12-28
001050632 915__ $$0StatID:(DE-HGF)1040$$2StatID$$aDBCoverage$$bZoological Record$$d2024-12-28
001050632 915__ $$0StatID:(DE-HGF)1060$$2StatID$$aDBCoverage$$bCurrent Contents - Agriculture, Biology and Environmental Sciences$$d2024-12-28
001050632 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2024-02-08T09:43:24Z
001050632 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2024-02-08T09:43:24Z
001050632 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2024-12-28
001050632 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2024-12-28
001050632 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2024-12-28
001050632 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2024-12-28
001050632 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2024-12-28
001050632 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2024-12-28
001050632 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
001050632 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2024-12-28
001050632 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
001050632 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2024-12-28
001050632 920__ $$lyes
001050632 9201_ $$0I:(DE-Juel1)IBG-2-20101118$$kIBG-2$$lPflanzenwissenschaften$$x0
001050632 980__ $$ajournal
001050632 980__ $$aVDB
001050632 980__ $$aUNRESTRICTED
001050632 980__ $$aI:(DE-Juel1)IBG-2-20101118
001050632 9801_ $$aFullTexts